Feline panleukopenia virus (FPV) causes fatal leukopenia and severe hemorrhagic diarrhea in cats. Although FPV isolates have been reported worldwide from several animals, the biological genetic features of South Korean FPVs remain unclear. We characterized molecularly isolates. Crandell-Rees feline kidney (CRFK) cells were used to isolate 60 organ homogenates. The confirmed be via analyses cyto...

FMR antigens are found on the surface of cells infected with Friend, Moloney, and Rauscher murine leukemia viruses (MuLV). These antigens are serologically distinct from the G cell surface antigens that are found on cells infected with endogenous MuLV (AKR and Gross virus). Cell surface antigens of both virus groups are immunogenic in mice, and immunization with appropriate virus-infected cells...

The targets for the immune system are antigens present on cancer cells; however, many are not cancer-specific and may also be found on normal tissues. These antigens are often products of mutated cellular genes, aberrantly expressed normal genes, or genes encoding viral proteins. Vaccines constitute an active and specific immunotherapy designed to stimulate the intrinsic antitumor immune respon...

In recent years, the use of viral vaccine vectors has gained immense interest over traditional vaccine strategies due to their ability to replicate in order to induce high immune responses against the intended antigens without the need for adjuvant co-administration. Several viral vectors, including but not necessarily limited to adenoviruses, poxviruses and alphaviruses are currently in variou...

We constructed a complete cDNA clone of strain GDVII of Theiler's virus in Bluescript plasmid. This recombinant plasmid, called pTMGDVII, was used to synthesize full-length RNA transcripts of the viral insert. This RNA was infectious for BHK cells. Virus R1-GDVII, obtained from transfected BHK cells, caused rapidly fatal encephalomyelitis in BALB/c mice. High amounts of viral antigens were pres...

The vertebrate immune system is a wonder of modern evolution. Occasionally, however, correlations within the immune system lead to inappropriate recruitment of preexisting T cells against novel viral diseases. We present a random energy theory for the correlations in the naive and memory T cell immune responses. The non-linear susceptibility of the random energy model to structural changes capt...

In this study, immunohistochemistry was used to clarify the early stages of viral kinetics and cycliccourse of IBDV, IR499, which has been described earlier as a very virulent strain (vvIBDV). Fifteen, 4-week-old SPF chickens were inoculated with 10³ EID50 of vvIBDV, IR499, via oculo/nasal route. Five birdsserved as controls, and inoculated with phosphate buffered saline (PBS). The birds were t...

A method was developed for directly observing the inner surfaces of plasma membranes by light and electron microscopy. Human erythrocytes were attached to cover slips (glass or mica) treated with aminopropylsilane and glutaraldehyde, and then disrupted by direct application of a jet of buffer, which removed the distal portion of the cells, thus exposing the cytoplasmic surface (PS) of the flatt...

Class I transplantation antigens form complexes with a virus protein encoded in the early region E3 of the adenovirus-2 genome. The interaction between this viral glycoprotein, E19, and nascent human class I antigens has been examined by microinjecting purified mRNA into Xenopus laevis oocytes. Both E19 and the two class I antigen subunits, the heavy chain and beta 2-microglobulin (beta 2M), we...