نتایج جستجو برای: viroid movement

تعداد نتایج: 157770  

Journal: :International Organization of Citrus Virologists Conference Proceedings (1957-2010) 1991

2014
Zhibo Zhang YeonKyeong Lee Carl Spetz Jihong Liu Clarke Qiaochun Wang Dag-Ragnar Blystad

Chrysanthemum stunt viroid (CSVd) is a damaging pathogen attacking Argyranthemum plants. Our study attempted to reveal distribution patterns of CSVd in shoot apical meristems (SAM) and to explore reasons for differential ability of CSVd to invade SAM of selected Argyranthemum cultivars. Symptom development was also observed on greenhouse-grown Argyranthemum plants. Viroid localization using in ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2004
Ming-Bo Wang Xue-Yu Bian Li-Min Wu Li-Xia Liu Neil A Smith Daniel Isenegger Rong-Mei Wu Chikara Masuta Vicki B Vance John M Watson Ali Rezaian Elizabeth S Dennis Peter M Waterhouse

Viroids and most viral satellites have small, noncoding, and highly structured RNA genomes. How they cause disease symptoms without encoding proteins and why they have characteristic secondary structures are two longstanding questions. Recent studies have shown that both viroids and satellites are capable of inducing RNA silencing, suggesting a possible role of this mechanism in the pathology a...

2009
Francesco Di Serio Andreas Gisel Beatriz Navarro Sonia Delgado Ángel-Emilio Martínez de Alba Giacinto Donvito Ricardo Flores

Northern-blot hybridization and low-scale sequencing have revealed that plants infected by viroids, non-protein-coding RNA replicons, accumulate 21-24 nt viroid-derived small RNAs (vd-sRNAs) similar to the small interfering RNAs, the hallmarks of RNA silencing. These results strongly support that viroids are elicitors and targets of the RNA silencing machinery of their hosts. Low-scale sequenci...

SAA Bagherian ,

Sweet lime split bark is a disorder of increasing importance in Fars province. Previously we showed that a new variant of hop stunt viroid (HSVd-sb) was related to the disorder. It had homology with a noncachexia variant of hop stunt viroid (CVd-IIa-117) which causes cracking the bark of trifoliate orange rootstocks. In this study, showed that M.NgoBIX, BglI and BsrI restriction enzymes can c...

Journal: :Japan Agricultural Research Quarterly: JARQ 2013

Journal: :Nucleic acids research 1991
C Davies C C Sheldon R H Symons

The plus and minus RNAs of the 247 nt avocado sunblotch viroid (ASBV) undergo site specific RNA self-cleavage reactions in vitro. As with several other self-cleaving RNAs, we proposed hammerhead secondary structures for the sequence around the site of self-cleavage of both RNAs. We have shown previously that, during transcription of a dimeric plus ASBV RNA, a double-hammerhead structure formed ...

Journal: :Virology 2008
Alberto Carbonell Angel-Emilio Martínez de Alba Ricardo Flores Selma Gago

Infection by viroids, non-protein-coding circular RNAs, occurs with the accumulation of 21-24 nt viroid-derived small RNAs (vd-sRNAs) with characteristic properties of small interfering RNAs (siRNAs) associated to RNA silencing. The vd-sRNAs most likely derive from dicer-like (DCL) enzymes acting on viroid-specific dsRNA, the key elicitor of RNA silencing, or on the highly structured genomic RN...

2014
Tamara Giguère Charith Raj Adkar-Purushothama Jean-Pierre Perreault

Viroids are small, circular, single stranded RNA molecules that infect plants. Since they are non-coding, their structures play a critical role in their life cycles. To date, little effort has been spend on elucidating viroid structures in solution due to both the experimental difficulties and the time-consuming nature of the methodologies implicated. Recently, the technique of high-throughput ...

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