In this work, cattle manure was diluted and pre-treated with biomass ash to yield a liquid fraction solid precipitate. Microalgae grown in the fraction, batch semi-continuous mode, achieved maximum productivities of 522.9 554.3 mg L−1 day−1 (12 days) for Chlorella protothecoides Tetradesmus obliquus, respectively. Nutrient removal efficiency highest semicontinuous mode replacement 10% reactor v...