نتایج جستجو برای: wnt3a protein

تعداد نتایج: 1235101  

2012
Miryam A. Fragoso Amit K. Patel Rei E. I. Nakamura Hyun Yi Krishna Surapaneni Abigail S. Hackam

Wnt/β-catenin signaling is an essential pathway that regulates numerous cellular processes, including cell survival. The molecular mechanisms contributing to pro-survival Wnt signaling are mostly unknown. Signal transducer and activator of transcription proteins (STATs) are a well-described family of transcription factors. STAT3 induces expression of anti-apoptotic genes in many tissues and is ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2008
David C Hay Judy Fletcher Catherine Payne John D Terrace Ronald C J Gallagher Jan Snoeys James R Black Davina Wojtacha Kay Samuel Zara Hannoun Anne Pryde Celine Filippi Ian S Currie Stuart J Forbes James A Ross Philip N Newsome John P Iredale

Human embryonic stem cells (hESCs) are a valuable source of pluripotential primary cells. To date, however, their homogeneous cellular differentiation to specific cell types in vitro has proven difficult. Wnt signaling has been shown to play important roles in coordinating development, and we demonstrate that Wnt3a is differentially expressed at critical stages of human liver development in viv...

2016
Shengtao Lin Tao Jiang Ling Ye Zhongbo Han Yuan Liu Chenchen Liu Chenwei Yuan Senlin Zhao Jian Chen Jingtao Wang Huamei Tang Su Lu Liguang Yang Xiaoliang Wang Dongwang Yan Zhihai Peng Junwei Fan

In this study, we aimed to elucidate the clinical significance and underlying mechanisms of BRG1 in colon cancer. In the clinical analysis, overexpression of BRG1 correlates with colon cancer progression in two cohorts (n = 191 and n = 75). Kaplan-Meier survival analysis revealed that BRG1 is a prognosis predictor for overall survival (P < 0.001) and disease-free survival (P = 0.001). Knocking ...

2015
Roberto Narcisi Mairéad A. Cleary Pieter A.J. Brama Martin J. Hoogduijn Nesrin Tüysüz Derk ten Berge Gerjo J.V.M. van Osch

Mesenchymal stem cells (MSCs) are a potential source of chondrogenic cells for the treatment of cartilage disorders, but loss of chondrogenic potential during in vitro expansion and the propensity of cartilage to undergo hypertrophic maturation impede their therapeutic application. Here we report that the signaling protein WNT3A, in combination with FGF2, supports long-term expansion of human b...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 2012
Xian-Zi Wan Bei Li Yi-Chen Li Xiang-Ling Yang Wei Zhang Ling Zhong Shao-Jun Tang

A disintegrin and metalloproteinase 10 (ADAM10) is the constitutive α-secretase that governs the nonamyloidogenic pathway of β-amyloid precursor protein processing and is an attractive drug target for treating Alzheimer's disease. To date, little is known about the mechanism by which ADAM10 is regulated in neurons. Using mouse primary cortical neurons, we show here that NMDA receptor (NMDAR) ac...

2014
Caixia Li Helmy M. Siragy

(Pro)renin receptor (PRR) expression is upregulated in diabetes. We hypothesized that PRR contributes to podocyte injury via activation of Wnt-β-catenin-snail signaling pathway. Mouse podocytes were cultured in normal (5 mM) or high (25 mM) D-glucose for 3 days. Compared to normal glucose, high glucose significantly decreased mRNA and protein expressions of podocin and nephrin, and increased mR...

Journal: :Blood 2013
Iain C Macaulay Jonathan N Thon Marloes R Tijssen Brian M Steele Bryan T MacDonald Gerardene Meade Philippa Burns Augusto Rendon Vishal Salunkhe Ronan P Murphy Cavan Bennett Nicholas A Watkins Xi He Desmond J Fitzgerald Joseph E Italiano Patricia B Maguire

Wnt signaling is involved in numerous aspects of vertebrate development and homeostasis, including the formation and function of blood cells. Here, we show that canonical and noncanonical Wnt signaling pathways are present and functional in megakaryocytes (MKs), with several Wnt effectors displaying MK-restricted expression. Using the CHRF288-11 cell line as a model for human MKs, the canonical...

Journal: :Journal of cell science 2010
Noriko Yokoyama Urszula Golebiewska Hsien-yu Wang Craig C Malbon

Dishevelled-3 (Dvl3) is a multivalent scaffold protein that is essential to Wnt signaling during development. Although Dvl-based punctae have been visualized by fluorescence microscopy; the physical nature and dynamic character of the such complexes are enigmatic. We use steric-exclusion chromatography, affinity pull-downs, proteomics and fluorescence correlation microscopy to characterize supe...

2017
Qiang Yang Wen-wen Wang Pu Ma Zhong-xuan Ma Meng Hao Temitope I Adelusi Lei-Du Xiao-Xing Yin Qian Lu

Objectives Increasing evidence suggests that regular physical exercise improves type 2 diabetes mellitus (T2DM). However, the potential beneficial effects of swimming on insulin resistance and lipid disorder in T2DM, and its underlying mechanisms remain unclear. Materials and Methods Rats were fed with high fat diet and given a low dosage of Streptozotocin (STZ) to induce T2DM model, and subs...

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