Thermophilic Bacillus sp. GUS1, isolated from  a soil  sample obtained from citrus garden, produced at least three proteases as detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and zymogram analysis. The enzymes were stable in the alkaline pH range (8.0-12.0), with the optimum temperature and pH range of the proteases being 70ºC and 6.0-12.0, respectively. All th...

A large number of studies on a lkaline phosphatase (AP) of chicken plasma have been made during the past forty years. Most of those studies are on the enzymic activity and the polymorphic studies are relatively few . In most of the polymorphic studies of chicken plasma AP, a starch gel electrophoresis has been employed. Major results by other investigators'"' may be summarized as follows. AP co...

Additional to industrial use of xylanase in kraft pulp production, thermostable xylanase has wider application in fishery, piggery, cattle food and human food. In this research, 1-4-β-Dendoxylanase was isolated from liquid state cultures of Bacillus brevis containing wheat straw as carbon source. Xylanase was purified to apparent homogeneity by gel filtration and ion exchange chromatography. Th...

Chitin is the second most abundant organic compound in nature. Although mesophilic bacteria degrade insoluble chitin, there a paucity of data describing degradation chitin by anaerobic thermophilic bacteria. In this report, we screened cow manure compost for new systems, and identified chitinolytic bacterial community (CBC) that showed high activity under conditions, i.e., 1% (w/v) powder degra...

Many reports highlight biological responses of crop plants after non-ionizing electromagnetic radiation (EMR) exposure based on the phenotypic and physiological levels. So, this study aimed to estimate genetic alterations in proteins, isozymes, DNA banding patterns as well extent nuclear damage economic corn (Zea mays) under stress EMR using accurate reliable bioassays like sodium dodecyl sulfa...

The rumen bacterium Cellulosilyticum ruminicola H1 efficiently hydrolyzes cellulose. To gain insights into the regulatory mechanisms of cellulase synthesis, comparative transcriptome analysis was conducted for cultures grown on 2% filter paper, 0.5 and 0.05% cellobiose, and 0.5% birchwood xylan. It was found that cellulose induced a majority of (hemi)cellulases, including 33 cellulases and a ce...

elm leaf beetle, xanthogaleruca luteola, is a serious pest (particularly at its larval stage) of elm trees in many areas. the existence of three important enzyms namely: ?-amylase, ?- glucosidase and ?-glucosidase in the midgut of the insect at its larval stage was investigated. optimum activity for ?-, ?-glucosidase and ?-amylase was recorded at phs 5.0, 6.0 and 6.0 against p-nitrophenyl-?-d-g...

We previously cloned cDNAs for all the members (A, B and C) of Xenopus aldolase gene family, and using in vitro transcribed RNAs as references, performed quantitative studies of the expression of three aldolase mRNAs in embryos and adult tissues. A Xenopus egg contains ca. 60 pg aldolase A mRNA and ca. 45 pg aldolase C mRNA, but contains only ca. 1.5 pg aldolase B mRNA. The percent composition ...

BACKGROUND Tenascin is a large extracellular matrix glycoprotein generally found in adult tissues undergoing active remodeling such as healing wounds and tumors. To determine the potential role of tenascin-C (TN-C) in the pathophysiology of atherosclerosis, we investigated the pattern of expression of TN-C in human coronary atherosclerotic plaques. METHODS AND RESULTS Immunohistochemical stai...