Coriolopsis rigida was isolated as an endophytic fungus from the medicinal plant Cochlospermum regium. The cultivated on solid media containing rice or pineapple crown bran for analyzing production of extracellular enzymes. activities lipase, β-xylosidase, pectinase, fructosyltransferase (FTase), invertase, α-amylase and total cellulolytic (FPase) were determined. showed positive activity all e...

Cyclodextrins (CDs) are cyclic oligosaccharides composed of α-d-glucose units linked by α-(1,4)-glucosidic bonds. α-CD, β-CD and γ-CD contain 6, 7 or 8 d-glucose units, respectively. CDs can bind to the active site pancreatic α-amylase competitively inhibit enzyme. Adding 0.100 mg CD/mg native wheat starch substantially reduces in vitro activity is a more potent inhibitor than α-CD γ-CD. 0.025,...

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-mediated RNA degradation is catalyzed by a type III system in the hyperthermophilic archaeon Sulfolobus solfataricus Earlier work demonstrated that the system can be engineered to target specifically mRNA of an endogenous host reporter gene, namely the β-galactosidase in S. solfataricus Here, we investigated the effect of single...

The medaka fish α-amylase was expressed and purified. The expression systems were constructed using methylotrophic yeast Pichia pastoris, and the recombinant proteins were secreted into the culture medium. Purified recombinant α-amylase exhibited starch hydrolysis activity. The optimal pH, denaturation temperature, and K(M) and V(max) values were determined; chloride ions were essential for enz...

Adult male and female cockroaches, Periplaneta americana (L.) were assayed for the presence of digestive enzymes in the fore-, midand hindgut regions. Activities of αamylase, β-amylase, γ-amylase, proteinase and lipase were detected in the three gut regions except for the absence of γamylase and lipase in female hindgut. The presence of these enzymes partly explains the polyphagous feeding habi...

Type II diabetes mellitus is a common disease in the world and characterized by hyperglycemia. Prevention of reducing hyperglycemia depends on inhibition α-amylase α-glucosidase enzymes. In this study, antidiabetic profiles Paliurus spina-christi Mill. fruit were investigated. The species used as an folk medicine Turkey. inhibitory effect studies conducted to prove effect. n-hexane sub-extract ...

Sea lettuce (Ulva pertusa) is a nuisance species of green algae that is found all over the world. East-Asian species of the marine gastropod, the sea hare Aplysia kurodai, shows a clear feeding preference for sea lettuce. Compared with cellulose, sea lettuce contains a higher amount of starch as a storage polysaccharide. However, the entire amylolytic system in the digestive fluid of A. kurodai...

The mashing step underpins the brewing process, during which endogenous amylolytic enzymes in malt, chiefly β-amylase, α-amylase, and limit dextrinase, act concurrently to rapidly hydrolyze malt starch fermentable sugars. With barley malts, is relatively straightforward, due part malted barley’s high enzyme activity, thermostabilities, gelatinization properties. However, beers also contain glut...

• First time HPTLC- Fusarium avenaceum bioassay using mycelium suspension HPTLC-EDA combined with HPTLC-DART-HRMS and HPTLC-ESI-HRMS Preparative-scale isolation of eight multipotent clerodane diterpenes cholinesterase, glucosidase, amylase microbial inhibitory potential compounds Giant goldenrod ( Solidago gigantea Ait.) root extract was screened for bioactive by high-performance thin-layer chr...

Saline–alkali stress is an important environmental factor affecting the growth and development of plants. Plants affected by saline–alkali can mitigate damage regulating content osmoregulatory substances such as soluble sugars. Elucidating regulatory mechanism changes in sugar fractions jujube fruits under crucial for fruit industry saline areas. In this study, we investigated effects on conten...