The objective of this work was to compare the usefulness of a randomly amplified polymorphic DNA (RAPD) assay to that of the ATB ID32C kit (bioMérieux, France) for identification of different species of Candida isolated from clinical specimens. The RAPD-PCR patterns obtained with OPE-18 primer for identification of clinical isolates were consistent, and the different independent assays revealed...

PCR-based DNA fingerprinting was used to characterize 48 clinical isolates of Yersinia enterocolitica. The samples were examined by random amplified polymorphic DNA (RAPD-PCR) and inter-repeat PCR (IR-PCR). IR-PCR with two enterobacterial repetitive intergenic consensus primers resulted in patterns which were poorly discriminated; 2 of 11 arbitrary primers (RAPD-PCR) provided sufficient discrim...

Wine quality is determined by the particular yeast strains prevailing at various stages of fermentation. Therefore, ability to make an easy, fast, and unambiguous discrimination yeasts strain level great importance. Here, tandem repeat-tRNA (TRtRNA) method with 5GAC or ISSR-MB primer sets random amplified polymorphic DNA (RAPD) analysis (GTG)3, R5, RF2 oligonucleotides were tested on non-Saccha...

Genetic relationships among 24 genotypes of Hyophila involuta collected from five different natural populations Mount Abu (Rajasthan) is analysed using RAPD and SSR markers. Based on efficiency parameters calculated for each marker system such as polymorphic information content (RAPD = 0.34; 0.66), index 2.78; 2.62) resolving power 8.13; 2.23), the shows higher values some indices but microsate...

CONTENTS SYNOPSIS INTRODUCTION WHAT ARE RAPDs Introduction Scoring RAPD Polymorphisms Sources of Polymorphism and Amplification Artifacts Population Genetic, Genealogical, and Phylogenetic Implications of Dominance and Cryptic Variation: A Preliminary Overview PROBLEMS WITH RAPD-PCR AND SOME REMEDIES Repeatability of Banding Patterns Within and Among Individuals Background Some Remedies Comigra...

We determined mammalian cell invasion and virulence gene (inlA, inlB, and actA) sequences of Listeria monocytogenes strains belonging to a molecular subtype (RAPD 9) that often persists in Danish fish-processing plants. These strains invaded human placental trophoblasts less efficiently than other L. monocytogenes strains, including clinical strains, and they carry a premature stop codon in inl...

The genetic diversity of 33 Nigerian Helicobacter pylori isolates were studied using RAPD, PCR-RFLP and Southern blot analysis of ureA or ureCD gene probes. RAPD was able to distinguish the following number of isolates using the primers 3880: 5'-AAGAGCCCGT-3' (28), 3881 :5'-AACGCGCAAC-3' (33) and OPH8 :5'-GAAACACCCC-3' (25). Southern blot analysis using the ureCD probe was also able to distingu...

The aim of this work was to study the genetic diversity among flue-cured tobacco cultivars. RAPD and AFLP analyses were used to assess the genetic similarity among selected accessions of flue-cured tobacco. Seventy eight RAPD and 154 AFLP polymorphic bands were obtained and used to assess the genetic diversity among 28 tobacco accessions. The cultivar relationships were estimated through the cl...

The random amplified polymorphic DNA (RAPD) assay was used to assess the level of DNA damage in various exposed and unexposed Culex quinquefasciatus larvae to acetone and chloroform extracts of Curcuma longa and Melia azedarach at different concentrations (6.25, 12.5 and 25 ppm). This is the first report of an analysis of genomic alterations in plant extracts-treated mosquito larvae using RAPD-...

In this paper, nine geographical isolates of Schistosoma mansoni (three from Egypt, three from Saudi Arabia and 3 from Puerto Rico) were studied at the genotype level by RAPD analysis with two arbitrary primers. The genetic distance was measured by the percentage of unshared bands. The RAPD results showed that the Egyptian strains were closely related to Saudi strains but Puerto Rico strains cl...