Sister chromatid exchange (SCE) frequency is widely used as an indicator of spontaneous chromosome instability. We investigated SCE frequency in the peripheral blood lymphocytes of familial and sporadic breast cancer (BC) patients from the Apulian Caucasian Population. Eighty-one patients were enrolled: 22 with familial history and 59 sporadic patients. Eleven familial patients had an 'increase...

BACKGROUND The analysis of sister chromatid exchange (SCE) is a cytogenetic technique used to show DNA damage due to an exchange of DNA fragments between sister chromatids. OBJECTIVE To determine whether HLA-B27 positive patients with ankylosing spondylitis (AS) were associated with higher SCE frequencies than patients without B27. METHODS Lymphocytes from 38 patients with AS (15 women, 23 ...

When Bloom syndrome (BS) cells labeled with bromodeoxyuridine (BrdUrd) for one round of DNA replication were fused with nonlabeled normal cells, the hybrid cells had a normal level of sister chromatid exchange (SCE) at the first mitosis after fusion. However, when normal cells treated with mitomycin C (MC) were fused with nontreated normal cells, the MC-induced SCE was not affected by fusion wi...

The aim of this study was to investigate the ability of Carbosulfan (the effective ingredient in Marshal, a carbamate insecticide/nematocide), Ethyl Carbamate (EC=carcinogen and Ethyl Methanesulfonat (EMS=mutagen) separately and in mixtures to induce sister chromatid exchange (SCE) in human lymphocytes and to nivestigate whether the test substances, as a mixture had any synergistic or antogonis...

Background: Arsenic is a well-documented human carcinogen widely distributed in the environment. Chronic exposure of humans to inorganic arsenicals causes many adverse health effects. The present work was conducted to evaluate the protective effect of Syzygium cumini seed extract (SCE) on arsenic-induced genotoxicity and hepatotoxicity in Wistar albino rats. Methods: Rats were randomly divide...

The homing endonuclease PI- Sce I consists of a protein splicing domain (I) and an endonucleolytic domain (II). To characterize the two domains with respect to their contribution to DNA recognition we cloned, purified and characterized the isolated domains. Both domains have no detectable endonucleolytic activity. Domain I binds specifically to the PI- Sce I recognition sequence, whereas domain...

Sister-chromatid exchanges (SCE) were studied in allium cepa L. meristematic cells at the second and third divisions after BrdUrd-substitution during just the first or during the second and third cycles, respectively. The observed SCE nonreciprocal/reciprocal ratios detected at the third division in both experiments, as well as comparison of the lowest SCE frequency observed per cycle and expre...

To assess the antimutagenic potentials of ginsenoside Rh2 (Rh2), its effects on the baseline and mitomycin C-induced sister chromatid exchange (SCE) were examined in human peripheral blood lymphocytes (PBLs). The SCE frequency in PBLs treated with various concentrations of Rh2 for 72 h was decreased in a dose-dependent manner and was significantly lower than the baseline levels at 1.0 x 10(-10)...

Ripasudil hydrochloride hydrate (K-115), a specific Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor, was the first ophthalmic solution developed for the treatment of glaucoma and ocular hypertension in Japan. Topical administration of K-115 decreased intraocular pressure (IOP) and increased outflow facility in rabbits. This study evaluated the effect of K-115 on monkey tra...

Spontaneous and mutagen induced sister chromatid exchange (SCE) frequencies have been studied in nine patients with multiple sclerosis and in nine age and sex matched healthy controls. The incidence of spontaneous SCE in lymphocytes of the MS patients was significantly greater, by about 50%, than in those of the control subjects. When exposed to mitomycin C (MMC) or ethyl methane sulfonate (EMS...