The isolation and sequence of a cDNA clone encoding the complete glyoxysomal malate dehydrogenase [gMDH; (S)-malate:NAD+ oxidoreductase, EC 1.1.1.37] of watermelon cotyledons are presented. Partial cDNA clones were synthesized in a three part strategy, taking advantage of the polymerase chain reaction technology with oligonucleotides based on directly determined amino acid sequences. Subsequent...

We have studied the significance of the N-terminal presequence of watermelon (Citrullus vulgaris) glyoxysomal malate dehydrogenase [gMDH; (S)-malate:NAD+ oxidoreductase; EC 1.1.1.37] in microbody targeting. The yeast Hansenula polymorpha was used as heterologous host for the in vivo expression of various genetically altered watermelon MDH genes, whose protein products were localized by immunocy...

A new methodology for Emergent System Identification is proposed in this paper. The new method applies the self-organizing Group Method of Data Handling (GMDH) functional networks, Particle Swarm Optimization (PSO), and Genetic Programming (GP) that is effective in identifying complex dynamic systems. The focus of the paper will be on how Particle Swarm Optimization (PSO) is applied within Grou...

Network intrusion detection has been an area of rapid advancement in recent times. Similar advances in the field of intelligent computing have led to the introduction of several classification techniques for accurately identifying and differentiating network traffic into normal and anomalous. Group Method for Data Handling (GMDH) is one such supervised inductive learning approach for the synthe...

Coagulation-flocculation is the most important parts of water treatment process. Traditionally, optimum pre coagulant dosage is determined by used jar tests in laboratory. However; jar tests are time-consuming, expensive, and less adaptive to changes in raw water quality in real time. Soft computing can be used to overcome these limitations. In this paper, multi-objective evolutionary Pareto op...

This paper presents the analysis of the stopping maneuver of the human driver by using a new three-dimensional driving simulator that uses CAVE, which provides stereoscopic immersive vision. First of all, the difference in the driving behavior between 3D and 2D virtual environments is investigated. Secondly, a GMDH is applied to the measured data in order to build a mathematical model of drivin...