Analysis of clones isolated from a cosmid DNA library indicates that the Serratia marcescens chromosome contains at least two genes, chiA and chiB, which encode distinct secreted forms of the enzyme chitinase. These genes have been characterized by inspection of chitinase activity and secreted proteins in Escherichia coli strains containing subclones of these cosmids. The two chitinase genes sh...

Investigations on the development of a biocontrol agent against the alarming stored food pest Corcyra cephalonica has witnessed noteworthy growth in current years from mainly physiological studies, to tackling fundamental queries concerning the underlying mode of action. Chitinase from Trichoderma viride, which has inauspicious effects on feeding behavior, development and biochemical parameters...

Sorghum serves as staple food for millions of people in Asia. It ranks as the sixth most planted crop and it is vulnerable to fungal diseases resulting in decreased grain quality and yield loss. Agrobacterium strains used were LBA4404 harbouring pcambia-ubi-chi11 (rice chitinase), EHA105 harbouring pcambia-ubi RC7 (rice chitinase) with bar gene and EHA105 harbouring pMKURF2 (rice chitinase gene...

Streptomyces coelicolor A3(2) has 13 chitinase genes encoding 11 family 18 and two family 19 chitinases. To compare enzymatic properties of family 19 chitinase and family 18 chitinases produced by the same organism, the four chitinases (Chi18bA, Chi18aC, Chi18aD, and Chi19F), whose genes are expressed at high levels in the presence of chitin, were produced in Escherichia coli and purified. The ...

Phylogeny-based analysis of chitinase and 16S rRNA genes from metagenomic data suggests that salinity is a major driver for the distribution of both chitinolytic and total bacterial communities in aquatic systems. Additionally, more acidic chitinase proteins were observed with increasing salinity. Congruent habitat separation was further observed for both genes according to latitude and proximi...

Inflammation plays a vital role at different stages of tumor progression. The development of tumors is affected by inflammatory mediators produced by the tumor and the host. YKL-40/chitinase-3-like-1 protein is often up-regulated in inflammation-associated diseases. With the use of chronic inflammatory disease systems, we describe the role of YKL-40/chitinase-3-like-1 protein in enhancing the i...

Chitin, after cellulose, the second most abundant biopolymer on earth, is a key component of insects, fungi, and house-dust mites. Lower life forms are endowed with chitinases to defend themselves against chitin-bearing pathogens. Unexpectedly, humans were also found to express chitinases as well as chitinase-like proteins that modulate immune responses. Particularly, increased levels of the ch...

one concern about using transgenic plants is the genetic variation that occurred from theirs tissue culture and regeneration. molecular markers are an important element for efficient and effective determination of genetic variation. the present work was carried out to assess the genetic uniformity of transgenic cottons (bt and chitinase lines), using rapd, issr molecular markers and sds-page an...

Abstract Chitin is abundant in nature and its degradation products are highly valuable for numerous applications. Thermophilic chitinases increasingly appreciated their capacity to biodegrade chitin at high temperatures prolonged enzyme stability. Here, using deep learning approaches, we developed a prediction tool, Preoptem, screen thermophilic proteins. A novel chitinase, Chi304, was mined di...

School of Biological Sciences, The Flinders University of South Australia, GPO Box 2100, Adelaide, SA 5001, Australia A cluster of three closely linked chitinase genes organized in the order chiA, chiB and chic, with the same transcriptional direction, and two unlinked genes, chip and chiQ, involved in chitin degradation in Pseudoalteromnas sp. strain S91 were cloned, sequenced and characterize...