Oxidative stress, defined as an increase in reactive oxygen species, has been implicated in a number of diseases, including hypertension. Superoxide is a particularly detrimental reactive oxygen species in the cardiovascular system because it reacts directly with NO, thereby limiting the amount of NO normally available for processes such as endothelium-dependent relaxation. Superoxide dismutase...

The objective of the present study was to investigate the effects of dietary supplemental Zinc (Zn) source and level on antioxidant ability and fat metabolism-related enzymes of broilers. Dietary treatments included the Zn-unsupplemented corn-soybean meal basal diet (control) and basal diets supplemented with 60, 120, or 180 mg Zn/kg as Zn sulfate, Zn amino acid chelate with a weak chelation st...

In the present study, we used the hph-1 mouse, which displays GTP-cyclohydrolase I (GTPCH I) deficiency, to test the hypothesis that loss of tetrahydrobiopterin (BH(4)) in conduit and small arteries activates compensatory mechanisms designed to protect vascular wall from oxidative stress induced by uncoupling of endothelial nitric oxide synthase (eNOS). Both GTPCH I activity and BH(4) levels we...

We investigated the effects of hypoxia/reoxygenation exposure on the barrier function of endothelial cell monolayers. Bovine pulmonary microvessel endothelial cells were grown to confluence on microporous filters (0.8-microns pore diameter) and exposed to hypoxia (0.1% O2 or PO2 approximately 1 mm Hg) for 2, 4, 12, or 24 hours, followed by reoxygenation with room air for a period ranging from 1...

Oxidative stress mediated by pro-oxidants has been implicated in the pathogenesis of vascular disorders. However, the effect of pro-oxidants on myogenic regulation of vascular function and the differential influence of gender is not known. Superoxide dismutase (SOD), an intracellular enzyme restricts excess pro-oxidant levels and may limit vascular dysfunction. We therefore tested the effects o...

Reactive oxygen species (ROS) have been implicated in the pathogenesis of many clinical disorders such as adult respiratory distress syndrome, ischemia-reperfusion injury, atherosclerosis, neurodegenerative diseases, and cancer. Genetically engineered animal models have been used as a tool for understanding the function of various antioxidant enzymes in cellular defense mechanisms against vario...

To examine effects of exogenous Cd on the kidney antioxidant defense system (AOS) and the possible protective role of Se against Cd toxicity, male Wistar albino rats (2 months old) were exposed during 30 days to oral intake of 200 ppm Cd (as CdCl2), 0.l ppm Se (as Na-selenite) or to the same doses of Cd / Se, simultaneously. Marked accumulation of Cd (23.44 +/- 0.69 micrograms/g w.m.) and marke...

هدف: از چالش­های عمده بشریت افزایش افسردگی و اختلال عملکرد جنسی ناشی داروهای ضدافسردگی است. با توجه به نقش سلول­های سرتولی در اسپرماتوژنز، پژوهش حاضر، اثر داروی دولوکستین را بر زنده­مانی، آپوپتوزیس بیان ژن­های Bax و­ (Connexin 43) Cx43 بررسی کرده مواد روش‌‌ها: TM4 محیط DMEM/F12 حاوی %5/2 FBS، 5% سرم اسب %1 پنی سیلین-استرپتومایسین کشت شدند. دوزهای 30،60، 15، 5/7، 75/3 میکرو­گرم/ میلی­لیتر زمان­ه...