L-Methionine gamma-lyase (MGL) catalyzes the pyridoxal 5'-phosphate (PLP) dependent alpha,gamma-elimination of L-methionine. We have determined two crystal structures of MGL from Pseudomonas putida using MAD (multiwavelength anomalous diffraction) and molecular replacement methods. The structures have been refined to an R-factor of 21.1% at 2.0 and 1.7 A resolution using synchrotron radiation d...

The second messenger hydrogen peroxide transduces changes in the cellular redox state by reversibly oxidizing protein cysteine residues to sulfenic acid. This signaling event regulates many cellular processes but has never been shown to occur in the brain. Here, we report that hydrogen peroxide heightens endocannabinoid signaling in brain neurons through sulfenylation of cysteines C201 and C208...

The present investigation was carried out to study the In vitro shoot multiplication and callogenesis in Gladiolus. For this purpose, cormel, meristem and leaf explants were chosen. Different concentrations of BAP i. e., 0.5, 1.0, 1.5, 2.0 or 2.5 mgL (2.22, 4.44, 6.66, 8.88 or 11.10 μM) were supplemented to MS medium. Both cormel and meristem explants showed maximum multiplication on MS medium ...

In Escherichia coli, several systems are known to transport glucose into the cytoplasm. The main glucose uptake system under batch conditions is the glucose phosphoenolpyruvate:carbohydrate phosphotransferase system (glucose PTS), but the mannose PTS and the galactose and maltose transporters also can translocate glucose. Mutant strains which lack the enzyme IIBC (EIIBC) protein of the glucose...

Cucumis melo L. is one of the most economically important horticultural crops worldwide. However,   low plant regeneration frequency of this plant during genetic transformation is  the  major hurdle for  applying biotechnological approaches. Hence, this study aimed to evaluate the effect of 6-benzyladenine (BA), cefotaxime (CTX), kanamycin (KAN), and indole-3-acetic acid (IAA) on the regenerati...

Thirty four arsenic resistant bacterial strains were isolated from the soil samples of Khulna Shipyard. They by growing them on nutrient broth medium containing 5 mgl-1 arsenic. From them, five finally selected, and studied their morphological, biochemical ecological characters in details. identified as Bacillus lichenifomis, polymyxa, Listeria murrayi, Moraxella urethralis Planococcus citreus....

Yolk-sac fry of Goldfish (Carassius auratus gibelio) were exposed to various concentrations of Al and pH for 3, 7 and 10 days. In this study number of dead fish, total length of larvae, yolk sac length, swimming activity, the number of gill cover movements and the number of heart beat was measured. The lowest and highest number of gill cover movements belong to the waters containing 50 mg L-1 o...

BACKGROUND Glycemic variability (GV) has recently been associated with mortality in critically ill patients. The impact of diabetes or its absence on GV as a risk factor for mortality is unknown. METHODS A total of 4084 adult intensive care unit (ICU) patients admitted between October 15, 1999, and June 30, 2009, with at least three central laboratory measurements of venous glucose samples du...

in the present study, an efficient protocol has been developed for callus induction and production of ra in callus culture of satureja khuzistanica for the first time. in vitro callus induction was achieved from young shoot tip explants cultured on ms and b5 media supplemented with different concentrations of iba (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins bap and kin...