background: recombinant monoclonal antibodies have been marketed in last three decades as the major therapeutic proteins against different cancers. however choosing a proper medium and supplements to reach the high expression is a challenging step. despite of commercial serum free and chemically defined media, there are still numerous researches seeking the optimum media to gain higher expressi...

results based on our results, mab against recombinant erns was produced and reacted successfully with recombinant and natural antigens. conclusions with regards to the role of erns in the identification of pi animals, it appears that erns recombinant antigen and the specific monoclonal antibodies produced against it may be suitable for developing bvdv laboratory diagnostic assays. objectives er...

background: the affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as elisa. affinity of most igg specific mabs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...

The immunoperoxidase staining of 84 primary invasive breast carcinomas with four monoclonal antibodies (BRST-1, HMFG1, EMA, B72.3) was evaluated by semiquantitative light microscopical examination and quantitative image analysis. Major differences in the staining of the tumours for each of the monoclonal antibodies was observed. Correlation between monoclonal antibody staining and patient age, ...

300 Letters to the Editor Table 1. Diagnostic criteria for Wladenström's macroglobulinemia and IgM monoclonal gammopathy of undetermined significance. Wladenström's macroglobulinemia a) IgM monoclonal gammopathy of any entity b) Lymphomatous bone marrow infiltration by lymphocytes with clonal plasmacytoid differentiation c) Intertrabecular bone marrow infiltration d) Immunophenotype lymphoplasm...

Background: Lung carcinoma is a multiple type cancer comprising of small cell and non-small cell carcinomas (NSCLC). For therapeutic and diagnostic purposes, serum monoclonal antibodies have been produced against lung cancer. Objective: To charac-terize a murine monoclonal antibody (ME3D11) reactive with human NSCLC. Methods: A murine monoclonal antibody (ME3D11) reactive with human NSCLC was s...

Serum syndecan-1 was investigated in 189 patients with newly diagnosed monoclonal proteinemia [the diagnoses were multiple myeloma (66), monoclonal gammopathies of undetermined significance (MGUS; n=54), provisional MGUS (no bone marrow examination performed; n=69)] and 36 controls. Syndecan-1 levels ranged widely between all diagnostic categories and were of limited discriminatory value (sensi...

Two stable monoclonal hybridoma cell lines secreting type-specific antibodies against the human papillomavirus type 1 (HPV-1) were isolated. The monoclonal antibodies detected HPV-1 antigens in frozen sections of HPV-1-induced warts, using immunofluorescence or immunoperoxidase techniques, and they reacted with HPV-1 particles in an immunodiffusion test. The two monoclonal antibodies recognized...

The condition, also known as benign monoclonal gammopathy, is characterized by the presence of a monoclonal immunoglobulin or light chain on im munofixation of the patient’s serum or urine. Such a diagnosis can only be considered if underlying causes, most notably multiple myeloma, are excluded (Appendix 1, available at www .cmaj .ca /lookup /suppl /doi:10.1503 /cmaj .122090 //DC1). Because mon...

Multiple pilus types from a uropathogenic strain of Escherichia coli O6, strain 6260, were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), high-pressure liquid chromatography, binding assays, and erythrocyte adsorption. In addition, monoclonal antibodies were raised against purified pili of E. coli 6260 and used for immunological characterization. SDS-PAGE...