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Quinupristin is a white to very slightly yellow, hygroscopic powder. It is a combination of three peptide macrolactones. The main component of quinupristin (>88.0%) has the following chemical name: N-[(6R,9S,10R,13S,15aS,18R,22S,24aS )-22-[p-(dimethylamino)benzyl]-6 ethyldocosahydro-10,23-dimethyl-5,8,12,15,17,21,24-heptaoxo-13-phenyl-18-[[(3S )-3 quinuclidinylthio] methyl]-12H-pyrido[2,1-f ]...
products and tryptic digestion and ‘mapping’ of the peptides (Sargent & Vadlamudi, 1968; Mainwaring, 1964), indicated that globin was a product of the wheat-germ system. This globin was shown to be a mixture of the embryonic P and E globins by use of [3H]globin markers in the peptide ‘mapping’. It appears from our findings that a t 12h of incubation in uitro the mRNA for globin is present, but ...
Data on zidovudine pharmacokinetics in children dosed using World Health Organization weight bands are limited. About 45 HIV-infected, Ugandan children, 3.4 (2.6-6.2) years, had intensive pharmacokinetic sampling. Geometric mean zidovudine AUC0-12h was 3.0 h.mg/L, which is higher than previously observed in adults, and was independently higher in those receiving higher doses, younger and underw...
Mice were used as quality control for functional assays, in order to discriminate between daily variability and true differences between dolphins analyzed at different times. Female B6C3F1 mice (M. musculus; Charles River Laboratories, MA) that were approximately 30 days-old were maintained at 18-26°C with relative humidity between 40 and 70%, and a light/dark cycle at 12h intervals. Animals we...
Expression kinetics of BHK21 cells transfected with two VEE replicons. (a) Replicons encoding constitutive EGFP and mKate were co-electroporated into BHK21 cells, and EGFP and mKate fluorescence levels were measured by flow cytometry at 3h, 6h, 12h, day 1, day 2, day 3, day 4, day 5, day 6, and day 7. (b) The percentage of EGFP/mKate double positive, EGFP single positive, mKate single positive,...
با توجه به نقش حائز اهمیت برهزایی در سوددهی پرورش دهندگان گوسفند، در مطالعه حاضر اثرات تزریق گنادوتروپین سرم مادیان آبستن (pmsg) به همراه هورمون محرک رشد فولیکولی (fsh) و هورمون آزادکننده گنادوتروپین (gnrh) بر رشد فولیکولی و برهزایی میشهای نژاد افشاری مورد بررسی قرار گرفت. چرخه فحلی 64 رأس میش افشاری چندبار زایش کرده با میانگین وزنی 5/2±65 با استفاده از سیدرهای پروژسترونی 14 روزه همزمانس...
A system using hepatocyte suspensions in vitro was developed for studying the synthesis of albumin, fibrinogen and transferrin. Conditions for optimum survival of the hepatocyte and for synthesis of these plasma proteins were defined for this system. These conditions included the use of horse serum (17.5 percent, v/v, heat-inactivated), an enriched medium (Waymouth's MB 752/1), an O2 tension of...
Maximum inhibition of phenylalanine hydroxylase activity in the liver (85%) and in the kidney (50%) of suckling rats required the administration of over 9 mumol of p-chlorophenylalanine/10g body weight. Despite the decrease in the total activity from 184 to 34 units per 10g body weight, the injection of as much as 26 mumol of phenylalanine was required for its concentration in plasma to be stil...
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