background:  aeromonas spp. can cause diarrhea and various infections in humans. access to rapid techniques with a high sensitivity and specificity is strongly needed for the identification of aeromonas species. the aim of this study was to evaluate two different methods including api 20e bacterial identification tests and the molecular detection using pcr primers specific for 16s-rrna and 23s-...

16S rRNA gene sequencing has been widely used for probing the species structure of a variety of environmental bacterial communities. Alternatively, 16S rRNA gene fragments can be retrieved from shotgun metagenomic sequences (metagenomes) and used for species profiling. Both approaches have their limitations-16S rRNA sequencing may be biased because of unequal amplification of species' 16S rRNA ...

This paper reports the first isolation and culture of Ehrlichia canis in Spain from a naturally infected dog using the DH82 cell line. After DNA extraction and PCR amplification, a nearly complete (1412bp) sequence of the 16S rRNA gene of the new E. canis strain was obtained. The GenBank accession number for the nucleotide sequence of this strain is AY394465. This sequence was aligned with the ...

early diagnosis of bacteremia and its complications is the most important part of care and management of the febrile patients. however, a majority of patients who appear to be clinically septic have negative blood culture. the use of polymerase chain reaction (pcr) techniques has allowed identifying the pathogenic organisms rapidly and accurately. the objective of this study was to investigate ...

It is difficult to distinguish between clinically significant slowly-growing, non-pigmented mycobacteria, notably to separate M. aviumand M. intracellulare from one another and from M. scrofulaceum strains. The purpose of this study was to evaluate the extent to which 16S rRNA sequencing could be used to highlight the taxonomic relationships of the mycobacterial strains, which are difficult to ...

The relatedness among 91 Enterococcus strains representing all validly described species was investigated by comparing a 1,102-bp fragment of atpA, the gene encoding the alpha subunit of ATP synthase. The relationships observed were in agreement with the phylogeny inferred from 16S rRNA gene sequence analysis. However, atpA gene sequences were much more discriminatory than 16S rRNA for species ...

Methanotrophic populations have been studied in Newport Bay estuary, Southern California. Environmental clone banks were generated for 16S rRNA genes specific to methanotrophs and for a diagnostic functional gene, pmoA, encoding a conserved subunit of the particulate methane monooxygenase. These clone banks contained sequences specific to types I and II methanotrophs typically found in aquatic ...

The 16S-23S rRNA intergenic spacer regions of 14 strains representing the 14 serovars of Ureaplasma urealyticum were amplified by PCR and sequenced for genetic differentiation between the two biovars Parvo and T960. Although the spacer region of the Parvo and T960 biovars comprised 302 nucleotides and lacked spacer tRNA genes, 15 nucleotides were different between the two biovars. The four nucl...

The diversity of bacterial communities at three sites impacted by acid mine drainage (AMD) from the Yinshan Mine in China was studied using comparative sequence analysis of two molecular markers, the 16S rRNA and gyrB genes. The phylogenetic analyses retrieved sequences from six classes of bacteria, Nitrospira, Alphaproteobacteria, Gammaproteobacteria, Deltaproteobacteria, Acidobacteria, and Ac...

We have determined and compared partial 16S rRNA sequences from 23 Lyme disease spirochete isolates and aligned these with 8 sequences previously presented. The 16S rRNA signature nucleotide compositions were defined for each isolate and compared with the genomic species signature nucleotide sets previously established. To identify positions truly indicative of species classification which coul...