Wetlands, including peatlands, are the main source of natural methane emission. Well-defined fen microsites have different methane emissions rates, but it is not known whether the methane-producing Archaea communities vary at these sites. Possible horizontal variations of communities, in a natural oligotrophic fen, were analysed by characterizing the methanogens from two well-defined microsites...

CONTEXT Gastric cancer is the second most common cause of cancer related death worldwide. Although Helicobacter pylori has been classified as a class I carcinogen, the presence of infection is not a factor that alone is able to lead to gastric cancer, and one of the possible explanations for this is the existence of different strains of H. pylori with different degrees of virulence. OBJECTIVE...

Three beagle dogs were inoculated with mice spleen/liver homogenate infected with Ehrlichia species detected from Ixodes ovatus (EIO) and one dog was used as a control. All three infected dogs did not show clinical signs of disease except for mild pyrexia throughout the 41-day study period. Splenomegaly was observed from Day 7 post-inoculation (p.i.) in two of the dogs. Hematological and bioche...

The genus Streptomyces under phylum actinobacteria has been recognized as a prolific source for the production of bioactive secondary metabolites. An actinobacterial strain designated as DST103 isolated from a wetland fresh water sediment of Tamdil Lake, Mizoram, Northeast, India was identified as Streptomyces cyaneofuscatus (KY287599) using 16SrRNA gene sequencing which shares 99.87% sequence ...

This investigation's goal was to find Pseudomonas aeruginosa virulence factors in canine wound infections. 100 samples were gathered and tested for eight variables utilizing biochemical molecular techniques this purpose. According the findings, women (72%) had highest isolation ratio. The found various ratios, with capsule being (16.6%) of them, amylase enzyme detected (3.3%), hemolysin (70%), ...

aim : thereby we aimed to detection of bacterial dna (bactdna) in sbp by polymerase chain reaction (pcr) and its prognostic relevance in cirrhotic patients with culture-negative non-neutrocytic ascites(cnnna).  background: approximately 60% of patients with spontaneous bacterial peritonitis (sbp) are ascites culture negative. methods : of each 77 patients with cirrhosis and ascites, two samples...

Abstract This study revealed the bacterial community structure and effects of carbon nitrogen on distribution pattern in sediments Chang’an section Zao River Xi’an. And we expect to provide a theoretical basis for biological monitoring bioremediation by this study. Therefore, based 16SrRNA high-throughput sequencing technology, analyzed characteristics carbon, Zaohe River. The results showed th...

Objective: This work aimed to enhance colony polymerase chain reaction (PCR) for the 16S rRNA of several Escherichia coli strains. Methods: The isolation E. is done from gut chicken and soil. Then, we optimized condition PCR amplification 16s ribosomal RNA. We successfully designed primer 3 RNA made dilution solution with grade water that 1:10. Moreover, finally, a 20 μL contains master mix our...

the presence of helicobacter pullorum in intestinal tract of broiler chickens may be a potential risk for human health. in this study, a total of 100 caecal samples of broilers carcasses from 20 flocks at a poultry abattoir in mashhad suburb were tested for the presence of h. pullorum using modified conventional culture method by combination of culturing on brucella sheep blood agar and a filte...

The microbial population of the uterus fluctuates during the estrous cycle. Microflora of uterus may affect the establishment and maintenance of pregnancy in bitches. The endoscopic samples obtained from the vagina and uterus of 20 female adult mixed‑breed dogs. The uterine lavage samples were prepared for cytology, bacterial (aerobic and anaerobic) and fungal cultures. Uterine tissue samples w...