The DNA in isolated chloroplasts was visualized by the fluorescent probe 4'6-diamidino-2-phenylindole (DAPI). When excited with light of 360 nm, the DNA-DAPI complex fluoresces brilliantly at 450 nm. Nuclei also fluoresce but their nucleoli do not. RNase and Pronase treatment of chloroplasts did not affect the fluorescence but both pre- and posttreatment of DAPI-stained chloroplasts with DNase ...

BACKGROUND We examined the association between the use of remote patient monitoring (RPM) of implantable cardioverter defibrillators (ICD) and all-cause mortality and rehospitalization among patients undergoing initial ICD implant. METHODS AND RESULTS A limited data set was constructed from Boston Scientific ALTITUDE Registry and National Cardiovascular Data Registry ICD Registry between Janu...

The PAM/Highwire/RPM-1 (PHR) proteins are signaling hubs that function as important regulators of neural development. Loss of function in Caenorhabditis elegans rpm-1 and Drosophila Highwire results in failed axon termination, inappropriate axon targeting, and abnormal synapse formation. Despite broad expression in the nervous system and relatively dramatic defects in synapse formation and axon...

Presynaptic terminals contain highly organized subcellular structures to facilitate neurotransmitter release. In C. elegans, the typical presynaptic terminal has an electron-dense active zone surrounded by synaptic vesicles. Loss-of-function mutations in the rpm-1 gene result in abnormally structured presynaptic terminals in GABAergic neuromuscular junctions (NMJs), most often manifested as a s...

Proponents of RPM argue that RPM helps to sustain a high level of service at the point of sale and that such a high level is efficient. This paper provides a simple model which leads to the following conclusions: 1) RPM may increase or decrease the level of service. 2) Whether the service level is more efficient under RPM does not depend on the fact that service increases due to RPM. It may be ...

Aluminium and its alloys belong to the most widely used metallic materials for industrial applications. One of the possible casting methods is twin-roll casting which produces sheets with high solid solution supersaturation. Decomposition of this supersaturated material during heat treatment was studied in aluminium alloys from the AW-3003 series – one standard grade and the other modified by a...

OBJECTIVES Aldosterone synthase produces aldosterone, which regulates electrolytes and thereby blood pressure. Polymorphisms in aldosterone-synthase gene (CYP11B2) may associate with heterogeneous aldosterone production and hypertension. Hence, we investigated -344T/C, Iw/Ic polymorphisms of CYP11B2, plasma renin activity (PRA) and aldosterone concentration (PAC). DESIGN AND METHODS Consecuti...

The filter cake, generated from the cold-purification stage in a zinc electrowinning plant and considered as a hazardous waste containing heavy metals, was characterized for its chemical and mineralogical compositions by inductively couple plasma-optical emission spectroscopy (ICP-OES), X-rays diffractometry (XRD) and scanning electron microscopyenergy dispersive X-rays spectrometry (SEM-EDS). ...

Caprylic acid (CA) as model reactant was selectively reduced in a flow-through reactor in hydrogen stream at 21 bar total pressure and 240-360 °C over alumina loaded with the adjacent Co, Ni, Cu host and In guest metals. The main target of this research is the recognition of efficient cobalt catalysts for carboxylic group hydroconversion compared to more familiar nickel and copper composites. T...

BACKGROUND Peridural fibrosis (PF) is a normal complication after lumbar surgery. It is a challenge for both surgeons and patients. Rapamycin (RPM), a novel antibiotic with anti-proliferative and immunosuppressive properties, has been shown to be effective in preventing uncontrolled scar proliferation diseases. The object of the present research was to investigate the effects of RPM on inhibiti...