Preimplantation mouse embryos exhibit growth at the blastocyst stage when there is a dramatic increase in protein content between days 4 and 5 after hCG treatment. This increse in protein synthesis requires the uptake of amino acids from the surrounding medium, but the consumption of individual amino acids present as a mixture has not been examined. We therefore measured the simultaneous deplet...

Unusual bile acids in umbilical cord blood and amniotic fluid of term newborns and in sera and urine from adult patients with cholestatic liver diseases were analyzed by use of gas-liquid chromatography-mass spectrometry. These bile acids were compared in order to elucidate possible similarities of bile acid metabolism between fetal and cholestatic liver. In both umbilical cord blood and amniot...

Sesamin is known as a specific inhibitor of delta 5-desaturation, the conversion from dihomo-gamma-linolenic acid (20:3(n=6)) to arachidonic acid (20:4(n-6)). In the previous paper, we reported that sesamin inhibited delta 5-desaturation of n-6 fatty acids in rat hepatocytes but not that of n-3 fatty acids, from 20:4(n-3) to 20:5(n-3). Then, we studied the effects of sesamin on delta 5-desatura...

Previous in vitro studies the have suggested anti-inflammatory properties of caffeine. Coffee is also rich phenolic acids with several potential health benefits (chlorogenic acids), mainly 5-caffeoylquinic acid (5-CQA). The aim present study was to determine effect caffeine and 5-CQA, using concentrations comparable typical human consumption, on LPS-induced cytokine production whole blood cultu...

Naturally occurring hydroxamic acids and hydroxamates are reviewed and classified according to the chemistry of the hydroxamic acid functions. The identity of the Fe(m) trihydroxamate albomycin ö2 with antibiotic Ro 5-2667 is established. TABLE OF CONTENTS 1. Monohydroxamic acids 1.1. Linear hydroxamic acids 1.1.1. Primary hydroxamic acids 1.1.2. Secondary hydroxamic acids 1.2. Cyclic hydroxami...

Palm oil is naturally red due to the high content of beta carotene. Most fatty acids in crude palm (CPO) are saturated acids, namely palmitic acid (32-59%), while that pure (PPO) linoleic (5-11%). Fatty function as a moisturizer for skin. This study aimed compare effectiveness lotion preparations emollients from and oil. The concentration CPO PPO lotions was 5 15% using water (O/W) base. based ...

The effect of side chain conjugation on 7-dehydroxylation of bile acids has been investigated. C24-bile acids and their glycine and taurine conjugates and keto bile acids were incubated with pure strains of Eubacterium sp. VPI 12708. Bile acids of the 5 alpha- or 5 beta-series with a free terminal carboxyl group and a 3 alpha, 7 alpha-dihydroxy system were very effectively 7 alpha-dehydroxylate...

Both bile acids and bile alcohols, a subclass of steroids, are made from cholesterol as its major end metabolites by the liver of vertebrates. Their conjugates are the major constituents of the bile and possess a special function as an aid to intestinal lipid digestion and absorption. The most common naturally occurring bile acids in mammals, birds, most snakes, and various teleostean fishes, a...

background: addition of amino acids to the culture medium is beneficial for embryonic development in many species. objective: the objective of this study was to investigate the effects of amino acids on the in vitro maturation and embryonic development of the bovine oocyte. materials and methods: bovine ovaries were collected from a local abattoir and brought into laboratory. cumulus-oocyte com...