A simple micropropagation method from shoot tip explants was reported here for Clitoria ternatea L., an economically important medicinal plant. High frequency of micro shoots were obtained from this explants on MS medium supplemented with various concentrations of Indole Acetic acid (IAA) (0.5 2.0 mg/l) and KN (0.5 -2.0 mg/l). Maximum number of shoots was obtained from shoot tip explants in the...

Withania somnifera (L.) Dunal is high demanding valuable medicinal plants which have pharmaceuticals properties, known for its with anolides. Nodal segment explants of W. somnifera when cultured on MS (Murashige and skoog’s, 1969) medium horizontally containing different concentration(1-3mg/l) of 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ) produced multiple shoots on both axil...

A successful protocol was developed for mass propagation of Lawsonia inermis Linn., an important medicinal plant. Multiple shoots were induced in apical and axillary meristems derived from mature explants of L. inermis on Murashige and Skoog (1962) medium supplemented with 0.25 mg/l 6-benzylaminopurine (BA), 0.25 mg/l Kinetin (Kn), 0.5 mg/l ascorbic acid and 3% (w/v) sucrose. The rate of multip...

Pelargonium quercetorum Agnew grows naturally in the Hakkari province of Türkiye. Although P. has potential use as a medicine and ornamental plant, it is especially used medicinal plant for cure various diseases by local people. In vitro tissue culture methods are favorable propagation, conservation, breeding plants. We aimed this study to achieve regeneration from different explant types. Seed...

A series of experiments were carried out in order to optimize a protocol for the direct organogenesis of Chilean red clover germplasm. A range of cultivars were used to analyze the effect of explant source (crown or stem meristems of vegetative plants), culture media and plant growth regulators. Our findings showed that stem meristems were easier to obtain, presented lower levels of contaminati...

Embryogenic cultures of Araucaria angustifolia were established in a BM liquid medium supplemented with 2 μM 2,4-dichlorophenoxyacetic acid, 1 μM 6-benzylaminopurine and 1 μM kinetin (BM2) and in a BM medium free of growth regulators (BM0). During 42 days in culture, the cell growth pattern of both cultures was similar. The pH of the culture medium of both BM0 and BM2 underwent progressive redu...

Cinchona officinalis is an important species from the Andean cloud forest that has a low regeneration rate in natural populations. In vitro of C. been successfully established but somaclonal variation was not evaluated. The pathway and number subcultures on were evaluated using six ISSR primers amplified 58 loci Inter Simple Sequence Repeats (ISSR). A dendrogram based Jaccard´s genetic distance...

ABSTRACT Lavender (Lavandula dentata) has several medicinal applications, both in the fresh form and after industrial processing. Productivity increase could be obtained through mass propagation, expanding possibility of obtaining raw material for production essential oil. The objective this work was to develop a micropropagation protocol from explants seeds. Aspects vitro germination, multipli...

Cymbidium erythrostylum Rolfe is one of the most beautiful species genus cymbidium which used as a cut flower or indoor plant. However, it was registered rare even in its original habitat. This study carried out to develop protocol for vitro propagation C. erythrostilum. We protosomes obtained by asymbiotic germination seed on 1/2 Murashige and Skoog nutrient medium (MS) supplemented with 1 mg/...