A cystatin capture enzyme-linked immunosorbent assay (ELISA) using recombinant Fasciola gigantica cathepsin L1 antigen was developed to detect specific immunoglobulin G (IgG) subclass antibodies (IgG1, IgG2, IgG3, and IgG4) and was evaluated for its diagnostic potential for human fasciolosis. In an analysis of the sera of 13 patients infected with F. gigantica, 209 patients with other parasitic...

introduction: binding a monoclonal antibody to tumor associated antigens is an effective method for cancer therapy because these agents can specifically target malignant cells, in fact monoclonal antibodies are effective agents for diagnosis, grading and treatment of different kinds of cancers. methods: in this research, a new monoclonal antibody against colon cancer cells was prepared and anti...

: With the popularity of neoadjuvant therapy as first-line treatment, especially for advanced squamous cell carcinoma (SCC), focus has become accurate individualized treatment. Specifically, toxic side effects traditional platinum-doublet chemotherapy are high, so treatment with pembrolizumab plus is safer and more effective. Pembrolizumab a humanized monoclonal IgG4 kappa anti-PD1 antibody. It...

conjugation of monoclonal antibodies to super paramagnetic nanoparticles is an effective method for cancer diagnosis and treatment. in this study the humanized anti her2/neu monoclonal antibody- herceptin- was conjugated to super paramagnetic iron oxide (spio) nanoparticles using edc method. the concentration of the conjugated antibodies was measured by bradford assay. the antibody-nanoparticle...

The levels of total of IgG, IgG1, IgG2, IgG3 and IgG4 were evaluated in 54 patients with chronic paracoccidioidomycosis (PCM) before, during and after treatment using an enzyme-linked immunosorbent assay with Mexo and recombinant Pb27 (rPb27) as the antigens. Mexo was effective in distinguishing PCM patients from individuals in the negative control group (NC) based on total IgG and rPb27 perfor...

in this study 19 blv infected and 2 healthy lymph nodes were analysed by western blotting to detect viral p 24 antigen . western blotting was carried out by rabbit anti p24 serum and p24 monoclonal antibody. rabbit serum in western blotting could detect 45, 50, 54, 58 and less than 19 kd bands of proteins and monoclonal antibody could detect 30, 32, 39, 45, 50 and 53 kd bands. our results indic...

Dear Sir, We read with great interest the paper of Gupta et al. concerning a case of hypocomplementaemic tubulointerstitial nephritis (TIN) [1]. Hypocomplementaemic immune complex TIN in the absence of autoimmune disorders is a rare condition, and only a limited number of cases have been reported, of which most have been labelled idiopathic [2]. However, in recent years, several case reports an...

Immunoglobulin G4-related disease (IgG4-RD) is a new disease category involving many organ systems, including the endocrine system in general and the thyroid in particular. Since an initial association was made between hypothyroidism and autoimmune (IgG4-related) pancreatitis, more forms of IgG4-related thyroid disease (IgG4-RTD) have been recognized. Four subcategories of IgG4-RTD have so far ...

BACKGROUND Autoimmune pancreatitis is an IgG4-related fibroinflammatory condition often associated with obstructive jaundice, as most lesions are located at the head of the pancreas. IgG4 level can help in the diagnosis, but it is normal in nearly 30% of affected patients. CASE REPORT A 55-year-old woman presented with a 5-month history of 20-pound unintentional weight loss and intermittent a...

The effect of host infection, chronic clinical disease, and transmission intensity on the patterns of specific antibody responses in Bancroftian filariasis was assessed by analyzing specific IgG1, IgG2, IgG3, IgG4, and IgE profiles among adults from two communities with high and low Wuchereria bancrofti endemicity. In the high endemicity community, intensities of the measured antibodies were si...