This study aimed to develop a cryopreservation protocol for the long-term preservation of yacon [Smallanthus sonchifolius (Poepp. and Endl.)], an Andean crop with high fructooligosaccharide content in its tuberous roots. Initially, was developed using clone originated from Ecuador classified as ECU 41. Osmotic dehydration apical buds (2–3 mm long) carried out by assessing two plant vitrificatio...

Previous studies have reported many discrepancies about the best type and concentration of cryoprotective agents (CPAs) and biological variability among various pre-antral follicle classes after cryopreservation of ovarian tissue. The aim of this study was to investigate the impacts of some synthetic polymers on histological characteristics of different types of pre-antral follicles after bovin...

a:4:{s:10:"Background";s:513:"Obesity has become a major health problem across the world. In women, it is known to cause anovulation, sub-fecundity, increased risk of fetal anomalies and miscarriage rates. However, in women going for assisted reproduction the effects of obesity on egg quality, embryo quality, clinical pregnancy, live birth rates are controversial To assess the effect of women's...

Background Follicle cryopreservation has been proposed as an alternative fertility preservation option. Follicles can be cryopreserved in intact ovarian tissue pieces or after isolation of individual follicles from the fresh ovarian tissue using enzymatic or mechanical techniques. Researchers have used different cryoprotectants and various techniques to improve cryopreservation. It is plausible...

OBJECTIVE To evaluate the effect of low-level laser irradiation on proliferation and viability of murine adipose-derived stem cells previously submitted to cryopreservation. METHODS Adipose-derived stem cells were isolated from inguinal fat pads of three mice, submitted to cryopreservation in fetal bovine serum with 10% dimethylsulfoxide for 30 days and then thawed and maintained in normal cu...