Single domain antibodies (sdAbs) are the recombinantly-expressed variable domain from camelid (or shark) heavy chain only antibodies and provide rugged recognition elements. Many sdAbs possess excellent affinity and specificity; most refold and are able to bind antigen after thermal denaturation. The sdAb A3, specific for the toxin Staphylococcal enterotoxin B (SEB), shows both sub-nanomolar af...

The HLA (Human Leucocyte Antigens) class I and class II polymorphism in a sample population of 250 Vojvodinians using standard microlymphocytotoxicity test was investigated. The antigens with the highest frequencies were: HLA-A2 (50.4%), A1 (24.1%) and A3 (22.7%); B35 (22.7%), B51(5) (16.9%) and B18 (15.6%); DR11 (5) (34.5%), DR7 (20.0%) and DR3 (19.3%); DQ1 (44.8%), DQ7 (3) (30.9%) and DQ2 (13...

I’ll now sketch a proof. It basically follows by by recalling the definition of the probability of an even conditioned on X2 = a2. We first note that the set of choices for (X 1, X2, 3) given that X2 = a2 is def Sa2 = {(X1, a2, X3) : (X1, a2) ∈ C12 and (a2, X3) ∈ C23} . Conditioning on X2 = a2, we obtain a sample chosen uniformly from Sa2 . Thus, for (a 1, a2, a3) ∈ Sa2 , 1 P [(X1, X2, X3) = (a...

We describe a series of exhaustive computer searches to determine the maximum number of words in ternary codes of length 10 and minimum distance 7, and to find all such optimal codes. We show that there are exactly 10 inequivalent (10, 14, 7) ternary codes. We prove A3(10, 7) = 14 by showing that none of the (10, 14, 7)3 codes can be extended to a (10, 15, 7)3 code, thereby also giving A3(11, 7...

This report provides an overview of the achievements of working group A3 for bringing personalization functionality to the Semantic Web. It continues the work started in the deliverable A3-D1 which gave an overview on personalization know-how of partners in A3. In the deliverable at hand, we report on our achievements on reasoning for personalization in the Semantic Web (see Section 2), and par...

The incidence of anthrax, which is caused by Bacillus anthracis, in the human and animal population of Mongolia has increased recently, and control of this infection is a nationwide concern. In this study, 29 isolates obtained from animals and various regions in Mongolia from 2001 to 2007 were analyzed by performing multiple-locus variable-number tandem-repeat analysis for 8 loci (MLVA-8) to un...

Cytotoxic T lymphocytes (CTL), specific for influenza A/X31 virus, were generated from human peripheral blood lymphocytes. These CTL lysed target cells that were infected with the same virus and that shared HLA A or B locus antigens. Minimal lysis was observed when HLA-D antigens were shared. Not all HLA A and B antigens were equally effective. Efficient lysis of target cells was seen when HLA ...

Immunological functions of mast cells remain poorly understood. Studies in Kit mutant mice suggest key roles for mast cells in certain antibody- and T cell-mediated autoimmune diseases. However, Kit mutations affect multiple cell types of both immune and nonimmune origin. Here, we show that targeted insertion of Cre-recombinase into the mast cell carboxypeptidase A3 locus deleted mast cells in ...

Cyclic nucleotide-gated (CNG) channels in retinal photoreceptors play a crucial role in vertebrate phototransduction. The ligand sensitivity of photoreceptor CNG channels is adjusted during adaptation and in response to paracrine signals, but the mechanisms involved in channel regulation are only partly understood. Heteromeric cone CNGA3 (A3) + CNGB3 (B3) channels are inhibited by membrane phos...

Antigen-specific immunotherapy may offer a unique approach to fight cancer. We have demonstrated that specific immunotherapeutic regimens involving recombinant melanoma antigen family A3 (MAGE-A3) and different immunostimulants exert clinical anticancer activity. In particular, the combination of recombinant MAGE-A3 and AS15, a multicomponent immunostimulant, was found to elicit robust antigen-...