Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chromatography. On western blot analysis, it was demonstrated that thiol-purified antigen showed a s...

Twenty-five wether lambs (34 +/- 0.9 kg) fitted with ruminal and abomasal infusion catheters were used in a completely randomized design to determine the effects of differing proportions of ruminal and abomasal casein infusion on N balance in lambs fed a high-concentrate diet (85% corn grain, 1.6% N; DM basis) for ad libitum intake. Wethers were infused with 0 (control) or 10.4 g/d of N from ca...

development, traditional dairy calf management programs have restricted the amount of liquid feed pre-weaning to about 10% of birth weight.[1] However, current research is focusing more on the effects of elevating the plane of nutrition for pre-weaned dairy calves. By increasing the volume of liquid fed pre-weaning to about 20% of birth body weight, the results have been higher average daily ga...

The bioavailability of rafoxanide was compared after intraruminal and intra-abomasal administration in healthy adult sheep (n = 6) in a single dose, 2 parallel group study at 7.5 mg/kg. Rafoxanide concentrations in plasma were measured by means of HPLC analysis. Primary pharmacokinetic parameters for bioavailability and disposition of rafoxanide in plasma for both routes of administration were ...

Serum pepsinogen activity was determined in the blood serum of 126 clinically healthy cows by the method of Paynter. After slaughter the cows studied were classified into three groups according to established macroscopic changes to the mucous membrane of the abomasum: a group without changes, a group with ulcers and a group with other changes. The prevalence of the appearance of abomasal ulcers...

Abomasal abnormalities impose economic constraints on sheep production, most of which are subclinical and cannot be easily detected by routine clinical examination. In this survey, morphopathological lesions of 100 abomasa of sheep slaughtered in Fars Province, southern Iran were studied from spring to autumn of 2011. Grossly, raised pale to whitish nodular areas to irregularly thickened mucosa...

Ruminal microbiota plays an important role in the conversion of plant lignans into mammalian lignans. The main mammalian lignan present in the milk of dairy cows fed flax products is enterolactone (EL). The objectives of the present study were to investigate the effects of abomasal infusion of flax oil on the metabolism of flax lignans and concentrations of EL in biological fluids of dairy cows...