This paper deals with a preliminary study of the nucleic acid fraction of ten representative strains of atypical acid-fast bacilli isolated from sputum and gastric contents of patients with proven or suspected tuberculosis (Loesch and Petrik, 1939). Strains Ya and Fe were isolated from cultures which also contained colonies of virulent human-type tubercle bacilli. Cultures from which the remain...

Introduction: Granulomatous lesions comprise a large family of disorders sharing a common histological denominator of formation of granulomas, either immune or non-immune category with tuberculosis being the most common cause in our country. Aims and Objectives: To study the spectrum of granulomatous lesions in a tertiary care hospital, to categorise tuberculous versus non-tuberculous causes an...

The first report in this series presented evidence to show that artifacts may arise from the mechanical manipulation of tubercle bacilli while they are being spread on a slide for staining (Yegian and Porter, 1944). During such treatment the normally acid-fast cells can be transformed into the minute non-acidfast rods and granules that have been frequently interpreted as immature forms in a rep...

The criteria for admission are extended for the cases of the risk of Mycobacterium tuberculosis (TB) transmission and of the possible risk of treatment failure. The criteria for discharging are consisted of the 3 factors, (1) symptoms (cough, fever, etc.) are free after the standard chemotherapy of more than 2 weeks, (2) three different sputum smears are negative for acid-fast bacilli (AFB) aft...

Cyclospora cayetanensis is a cause of prolonged diarrhoea, mainly in travellers. Laboratory diagnosis may be achieved by a number of methods such as the staining of faecal smears by the modified Ziehl-Neelsen (ZN) technique. Safer methods using this technique have been described for the staining of acid fast bacilli and cryptosporidia by replacing the phenol content of the carbol fuschin stain ...

Despite the recent development of more sensitive technologies, diagnosis of tuberculosis in resource limited settings continues to rely on sputum smear microscopy. This is explained by the fact that smear microscopy is simple, inexpensive, and the most accessible tool in resource limited settings for tuberculosis diagnosis. Hot Ziehl-Neelsen (Z-N) is still used in laboratories of resource limit...

Background. Tuberculosis lymphadenitis is one of the most common forms of all extrapulmonary tuberculosis. Objective. To evaluate the magnitude of M. tuberculosis from lymph node biopsy paraffin-embedded sections among suspected patients visiting the Jimma University Specialized Hospital. Method. A cross-sectional study design of histological examination among lymph node biopsy paraffin-embedde...

A 71-year-old woman with polymyositis presenting with left thigh pain and an intermittent fever was admitted to Osaka Rosai Hospital. We initially diagnosed that her pain and fever were caused by a soft tissue infection because her polymyositis was controlled. She did not respond to various antibiotic therapies. Chest computed tomography demonstrated miliary tuberculosis (TB). Ziehl-Neelsen sta...

Annual incidence rates of extrapulmonary tuberculosis have been increasing over the last few years in the Kingdom of Saudi Arabia. True rates may even be higher due to incomplete reporting. Diagnosis of this condition requires high clinical suspicion, special diagnostic procedures, special staining, and culture media for acid fast bacilli. Delayed diagnosis results in increasing morbidity, mort...

Primary tuberculosis (TB) of the hard palate is very rare. A 74-year-old man was presented with 6-month history of dysphagia along with an irregular mass in the hard and soft palate. Magnetic resonance imaging (MRI) revealed thickened and increased signal intensity within hard and soft palate. Tissue biopsy showed focal caseating granulomatous-like lesion and the histochemical staining using Zi...