Protein phosphatase 5 (Ppp5) is a serine/threonine protein phosphatase comprising a regulatory tetratricopeptide repeat (TPR) domain N-terminal to its phosphatase domain. Ppp5 functions in signalling pathways that control cellular responses to stress, glucocorticoids and DNA damage. Its phosphatase activity is suppressed by an autoinhibited conformation maintained by the TPR domain and a C-term...

The radioimmunoassay of human prostate-specific acid phosphatase and the measurement of the catalytic activity of acid phosphatase using p-nitrophenyl phosphate as substrate were compared in the diagnosis and follow-up of therapy of prostatic cancer patients. We monitored 17 patients without metastases and eight patients with metastases for 12 months. We detected elevation of the catalytic acti...

The acid phosphatase activity of rat liver homogenates and of nuclear and cytoplasmic fractions isolated therefrom (by differential centrifugation) was determined biochemically in a series of experiments. For each liver, Gomori's histochemical test for acid phosphatase was run in parallel. No correlation was found between the biochemical and the histochemical results. According to the former, t...

Acid phosphatases are enzymes capable of hydrolyzing orthophosphoric acid esters in an acid medium. Prostatic acid phosphatase has served as a tumor marker for metastatic prostate cancer for many years. We have cloned a new human acid phosphatase gene (named testicular acid phosphatase, ACPT), which is expressed mainly in testis and to a lower extent in the prostate, trachea, and other tissues....

The protein serine/threonine phosphatases constitute a unique class of enzymes that are critical regulatory enzymes as they must counteract the activities of thousands of protein kinases in human cells. Uncontrolled inhibition of phosphatase activity by toxic inhibitors can lead to widespread catastrophic effects. Over the past decade, a number of natural product toxins have been identified whi...

Since the studies of Gutman and Gutman (2, 3), measurements of the serum acid phosphatase have become a recognized and widely used ancillary procedure for the diagnosis of cancer of the prostate and a practical means for the evaluation of the functional status of the tumor in many patients with metastasizing cancer of the prostate. While an abnormally increased value of serum acid phosphatase i...

In 1938, Gutman et al. (6) described increased levels of acid phosphatase in metastatic carcinoma of the prostate. Since then, when most of us hear the term "acid phosphatase" we immediately think of prostatic cancer, and justifiably so. The link, however, between the test and the diagnosis is not as clear and simple as many of us would like to believe. I would first like to discuss this enzyme...

Rat liver acid phosphatase (EC 3.1.3.2) was separated into two highly purified fractions, differing in isoelectric point and Km. One fraction was crystallized and proved homogeneous by ultracentrifugation and polyacrylamide gel electrophoresis. The molecular weights (lOO,OOO), substrate specificities, and pH optima of both enzymes were similar. Oxalate was a mixed type inhibitor to both enzymes...

Decryptification of acid phosphatase in Geotrichum sp. arthrospores was accomplished using acetone or dimethyl sulfoxide treatment. Both dimethyl sulfoxide and acetone irreversibly destroyed the integrity of the spore membranes without solubilizing acid phosphatase.

  The distribution and localization of acid and neutral mucins in various cells lining the olfactory epithelium of Cyprinus carpio have been studied histochemically by employing the PAS-AB technique. Variations in the localization of protein in different cells lining the olfactory epithelium have been correlated with the functional significance of the region concerned. Intense localization of t...