Bis-triazinyl-pyridines (BTPs) have been developed at CEA-Marcoule to separate actinides(III) from lanthanides(III). Although high separation performances were observed in the counter-current hot test carried out in 1999 on a highly active effluent, the extraction and back-extraction yields of actinides(III) were lower than expected from calculation. A strong sensitivity of the extractant towar...

The aim of this study was to establish the most efficient extraction conditions of betanin and to evaluate the termal stability of this red pigment in aqueous solution. The extraction of betanin from red beet (Beta vulgaris L.) was carried out under different conditions, varying the extraction parameters such as time and solid to solvent ratio, in acidic aqueous medium. The yields of the extrac...

At present, designing novel ligands for efficient actinide extraction in spent nuclear fuel reprocessing is extremely challenging due to the complicated chemical behaviors of actinides, the similar chemical properties of minor actinides (MA) and lanthanides, and the vulnerability of organic ligands in acidic radioactive solutions. In this work, a quantum chemical study on Am(III), Cm(III) and E...

The broad-spectrum antibiotic 2,4-diacetylphloroglucinol (Phl) is a major determinant in the biological control of a wide range of plant diseases by fluorescent Pseudomonas spp. A protocol was developed to readily isolate and quantify Phl from broth and agar cultures and from the rhizosphere environment of plants. Extraction with ethyl acetate at an acidic pH was suitable for both in vitro and ...

We have isolated a full-length cDNA clone from Arabidopsis thaliana that has extensive homology to p40 proteins from other organisms. The cDNA predicts a protein (A-p40) of 298 amino acids with a calculated molecular mass of 32.5 kDa and a pI of 4.8. Antibodies raised against an A-p40 fusion protein detected a polypeptide of about 40 kDa in Arabidopsis and about 42 kDa in corn and soybean. In t...

A mixed-mode solid-phase extraction procedure was developed for the isolation and purification of acidic compounds from complex biological matrices. Urine samples were spiked with several acidic drugs and diluted in ammonium acetate buffer. Isolute HAX columns (a mixed-mode phase consisting of both hydrophobic and ion-exchange ligands) were conditioned with methanol and ammonium acetate prior t...

The ternary mixture, heptane-isopropyl alcohol-water, provides a convenient two-phase system for extraction of longchain fatty acids (1). When the solvent components are taken in suitable proportions, the phases separate rapidly without centrifugation. The long-chain fatty acids distribute predominantly into the upper, nonpolar phnsc, whereas the more polar acids remain below. This method of ex...

A novel simultaneous phase-trafficking approach using spatially separated solid-supported reagents for rapid separation of neutral, basic, and acidic compounds from organic plant extracts with minimum labor is reported. Acidic and basic ion-exchange resins were physically separated into individual sacks ("tea bags") for trapping basic and acidic compounds, respectively, leaving behind in soluti...

The aim of this study was to increase the yield of biodiesel produced by Scenedesmus sp. through in situ transesterification by optimizing various process parameters. Based on the orthogonal matrix analysis for the acidic catalyst, the effects of the factors decreased in the order of reaction temperature (47.5%) > solvent quantity (26.7%) > reaction time (17.5%) > catalyst amount (8.3%). Based ...

the major objectives of this study were to find out the mechanism behind the sapstaining fungal hyphae penetration into wood elements using electron microscope and to evaluate the exact role of phenolic compounds existing in wood vinegar towards the sapstaining fungal growth inhibition. to formulate the new environmental friendly wood preservative, we neutralized (to ph 7) wood vinegar with nao...