نتایج جستجو برای: acrab tolc efflux pump

تعداد نتایج: 78937  

Journal: :Antimicrobial agents and chemotherapy 2001
O Lomovskaya M S Warren A Lee J Galazzo R Fronko M Lee J Blais D Cho S Chamberland T Renau R Leger S Hecker W Watkins K Hoshino H Ishida V J Lee

Whole-cell assays were implemented to search for efflux pump inhibitors (EPIs) of the three multidrug resistance efflux pumps (MexAB-OprM, MexCD-OprJ, MexEF-OprN) that contribute to fluoroquinolone resistance in clinical isolates of Pseudomonas aeruginosa. Secondary assays were developed to identify lead compounds with exquisite activities as inhibitors. A broad-spectrum EPI which is active aga...

2016
Ewa Otręebska-Machaj Jacqueline Chevalier Jadwiga Handzlik Ewa Szymańska Jakub Schabikowski Gérard Boyer Jean-Michel Bolla Katarzyna Kieć-Kononowicz Jean-Marie Pagès Sandrine Alibert

Multidrug resistant (MDR) bacteria are an increasing health problem with the shortage of new active antibiotic agents. Among effective mechanisms that contribute to the spread of MDR Gram-negative bacteria are drug efflux pumps that expel clinically important antibiotic classes out of the cell. Drug pumps are attractive targets to restore the susceptibility toward the expelled antibiotics by im...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2015
Jessica M A Blair Vassiliy N Bavro Vito Ricci Niraj Modi Pierpaolo Cacciotto Ulrich Kleinekathӧfer Paolo Ruggerone Attilio V Vargiu Alison J Baylay Helen E Smith Yvonne Brandon David Galloway Laura J V Piddock

The incidence of multidrug-resistant bacterial infections is increasing globally and the need to understand the underlying mechanisms is paramount to discover new therapeutics. The efflux pumps of Gram-negative bacteria have a broad substrate range and transport antibiotics out of the bacterium, conferring intrinsic multidrug resistance (MDR). The genomes of pre- and posttherapy MDR clinical is...

Journal: :The Journal of antimicrobial chemotherapy 2006
Ryoichi Saito Kenya Sato Wakako Kumita Natsuko Inami Hiroyuki Nishiyama Noboru Okamura Kyoji Moriya Kazuhiko Koike

OBJECTIVES We conducted a study to determine the role played by amino acid mutations in DNA gyrase and topoisomerase IV, and the AcrAB efflux pump in resistance to fluoroquinolones in clinical isolates of Proteus mirabilis. METHODS Nine clinical isolates of P. mirabilis containing eight fluoroquinolone-resistant isolates and one fluoroquinolone-susceptible isolate as the causative pathogen we...

2014
Toyotaka SATO Shin-ichi YOKOTA Risa ICHIHASHI Tomoka MIYAUCHI Torahiko OKUBO Masaru USUI Nobuhiro FUJII Yutaka TAMURA

Understanding the prevalence of antimicrobial-resistance and the relationship between emergence of resistant bacteria and clinical treatment can facilitate design of effective treatment strategies. We here examined antimicrobial susceptibilities of Escherichia coli isolated from dogs admitted to a university hospital (University hospital) and companion animal clinics (Community clinics) in the ...

Journal: :International journal of antimicrobial agents 2011
Mark I Garvey M Mukhlesur Rahman Simon Gibbons Laura J V Piddock

It was hypothesised that extracts from plants that are used as herbal medicinal products contain inhibitors of efflux in Gram-negative bacteria. Extracts from 21 plants were screened by bioassay for synergy with ciprofloxacin against Salmonella enterica serotype Typhimurium, including mutants in which acrB and tolC had been inactivated. The most active extracts, fractions and purified compounds...

2009
Jin-hui Hou Yong-hua Hu Min Zhang Li Sun

To date several classes of multidrug exporters have been identifi ed in prokaryotic cells, one of which is the resistance nodulation division (RND) family transporters (Poole and Srikumar, 2001). In this type of effl ux system, RND, an inner membrane proton-drug antiporter, forms a tripartite construct with a channel-forming outer membrane protein and an inner membrane protein of the MFP (membr...

Journal: :Antimicrobial agents and chemotherapy 2015
Jean-Marie Pagès Sabine Peslier Thomas A Keating Jean-Philippe Lavigne Wright W Nichols

This study examined the activity of the novel antimicrobial combination ceftazidime-avibactam against Enterobacteriaceae exhibiting different outer membrane permeability profiles, specifically with or without porins and with or without expression of the main efflux pump (AcrAB-TolC). The addition of the outer membrane permeabilizer polymyxin B nonapeptide increased the antibacterial activities ...

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