Since previous investigations have provided evidence that certain proteinases of the trophoblast playa key role in initiation of implantation of the embryo in the uterus, enzymic characterization of endopeptidase activity of rabbit blastocyst extracts was attempted. Recently developed tripeptide p-nitroanilide substrates now allow far the first time quantitative assays of enzyme activities in t...

OBJECTIVE To assess the contraceptive properties, antimicrobial activity, and safety of mandelic acid condensation polymer (SAMMA). DESIGN Experimental study of SAMMA's in vitro and in vivo properties. SETTING Academic research laboratories. PATIENT(S) Healthy volunteers for semen donation in an academic research environment. INTERVENTION(S) Inhibition of sperm function indicators, conc...

introduction: the objective of this study was to evaluate the relationship between spermiogenic defects such as protamine deficiency, and acrosomal integrity with fertilization and pregnancy rate in ivf patients. material and methods: density, motility and morpology of semen samples from 70 infertile couples undergoing ivf at isfahan fertility and infertility center were analyzed according to w...

We have used DNAse I footprinting to examine the formation of intermolecular DNA triple helices at sequences containing adjacent blocks of purines and pyrimidines. The target sites G6T6.A6C6 and T6G6.C6A6 were cloned into longer DNA fragments and used as substrates for DNAse I footprinting, which examined the binding of the acridine (Acr)-linked oligonucleotides Acr-T5G5 and Acr-G5T5 respective...