background: in nature, there are herbal extracts, while capable of reducing aflatoxin b1 in agricultural product by different mechanisms, may also act as growth promoters. objectives: in this study, aqueous extract of t. daenensis was evaluated to detoxify contaminated feed with aflatoxin b1 and to determine the effect of growth promoters in japanese quail. methods: to this purpose, at 24 d of ...

In Portugal, there is rather little information about the natural occurrence of aflatoxin in feedstuffs. The aim of this work was to report the results of screening the natural incidence of aflatoxin B1 (AFB1) in samples of cattle feed collected from seven dairy cow's farms from Portugal distributed in several locations of the country. One thousand and one samples were taken from 1995 to 2004. ...

A survey was conducted in the five rice-growing regions in Guyana to determine the presence of aflatoxins in multiple fractions of rice in June/October 2015 growing season. The fractions were paddy, steamed paddy, cargo rice, white rice and parboiled rice. Samples were analyzed by High Performance Liquid Chromatography. A subset of the samples was further analyzed by enzyme-linked immunosorbent...

background: due to the high toxicity of aflatoxin and its effects on public health, determination of aflatoxin level in wheat flour samples in the golestan province, north of iran was investigated. to examine the effect of seasonal changes, summer and winter sampling was performed with standard sampling methods. methods: a total of 200 flour samples were collected from 25 factories. hplc method...

The multidrug resistance (mdr) genes encode P-glycoproteins, integral membrane proteins which function as drug efflux transporters. Exposure of animals in vivo and cells in vitro to a variety of xenobiotics leads to increased mdr1 gene expression and higher levels of P-glycoprotein. This response may protect cells from the cytotoxic effects of these compounds. In this investigation we functiona...

Citation: Aswani Kumar YVV, Renuka RM, Achuth J, Venkataramana M, Ushakiranmayi M and Sudhakar P (2018) Development of Hybrid IgG-Aptamer Sandwich Immunoassay Platform for Aflatoxin B1 Detection and Its Evaluation Onto Various Field Samples. Front. Pharmacol. 9:271. doi: 10.3389/fphar.2018.00271 Development of Hybrid IgG-Aptamer Sandwich Immunoassay Platform for Aflatoxin B1 Detection and Its E...

The effect of rumen microbes on six mycotoxins (aflatoxin B1, ochratoxin A, zearalenone, T-2 toxin, diacetoxyscirpenol, and deoxynivalenol ) considered to be health risks for domestic animals was investigated. The mycotoxins were incubated with intact rumen fluid or fractions of rumen protozoa and bacteria from sheep and cattle in the presence or absence of milled feed. Rumen fluid had no effec...

A collaborative study was conducted to evaluate the effectiveness of an immunoaffinity column cleanup liquid chromatography (LC) method for determination of aflatoxin B1 in cattle feed at a possible future European regulatory limit (1 ng/g). The test portion was extracted with acetone-water (85 + 15), filtered, diluted with water, and applied to an immunoaffinity column. The column was washed w...

Background & Aims: Aflatoxins are a group of toxic compounds found in most plant products such as pistachio, corn, spices, wheat, and rice. This study was carried out to detect the presence of aflatoxin B1 (AFB1) in samples of spices collected from Qaemshahr in Mazandaran province, Iran. Methods: This was a cross-sectional study on the presence of AFB1 in 18 samples of spices including chilli (...

A high-affinity IgM monoclonal antibody specific for aflatoxins was covalently bound to Sepharose 4B and used as a preparative column to isolate aflatoxin derivatives from the urine of people and experimental animals who had been exposed to the carcinogen environmentally or under laboratory conditions. Aflatoxin levels were quantified by radioimmunoassay and high-performance liquid chromatograp...