نتایج جستجو برای: affinity separation
تعداد نتایج: 207918 فیلتر نتایج به سال:
An affinity-chromatographic method for determination of glycosylated hemoglobin (Anal. Lett. 14: 649-661, 1981) is compared with the thiobarbituric acid colorimetric (I) (Clin. Chem. 27: 669-672, 1981) and the ion-exchange liquid-chromatographic (II) (Diabetes 29: 623-628, 1980) methods. A correlation of 0.98 was obtained for the affinity method vs II and 0.97 for affinity vs I (n = 51). The wi...
Affinity Selection of Peptide Binders with Magnetic Beads <i>via</i> Organic Phase Separation (MOPS)
Novel immobilized Cu+2 ion grafted cellophane membranes for affinity separation of His-Tag Chitinase
Structural changes at the active site of an enzyme induced by binding to a substrate molecule can result in enhanced activity biological systems. Herein, we report that new hybrid ultramicroporous material sql-SIFSIX-bpe-Zn exhibits fit mechanism when exposed acetylene, C2H2. The resulting phase change affords exceptionally strong C2H2 turn enables highly selective C2H2/C2H4 and C2H2/CO2 separa...
A general strategy and a specific tactic for affinity purification of polypeptides synthesized on solid supports are desbribed and demonstrated. The desired peptide chains were distinguished from terminated peptide chains before removal from the support by attachment of an affinity reagnet (cysteinyl-methionine) bearing an affinity group (thiol) and a binding group (carboxylic acid). After clea...
Two distinct thyrotropin (TSH) binding species have been separated from solubilized porcine thyroid membranes. Membranes was solubilized with 1% Triton X-100, and the supernatant was recovered by centrifugation at 105,000 X g. Scatchard analysis of thyrotropin binding to solubilized membranes (SM) yielded a nonlinear plot with Kd values for the high and low affinity components similar to those ...
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