Aflatoxin B1 (AFB1) was detected in serum samples of healthy Japanese males by radioimmunoassay and high-performance liquid chromatography. Blood samples were obtained from 20 subjects after fasting and from 80 subjects after lunch. The subjects ranged in age from 20 to 63 years of age. Measurement of AFB1 in the samples was performed by radioimmunoassay and was confirmed by high-performance li...

This work describes the investigation of a label-free immunosensor for the detection of aflatoxin B1 (AFB1). CD-trodes (electrodes obtained from recordable compact disks) were used as low-cost and disposable transducers after modification with a self-assembled monolayer (SAM) of lipoic acid. The anti-aflatoxin B1 antibody was immobilized via EDC/NHS activation, followed by blocking with bovine ...

Two experiments were performed to screen bacilli isolated from quails for their aflatoxin removal potential and to assess the efficiency of their amelioration of experimental aflatoxicosis. Nonhemolytic bacilli were selected for in vitro aflatoxin B1 (AFB1) removal and conventional probiotic tests. The isolate with the highest scores was selected for assessment in field experiments and was iden...

mycotoxins are secondary metabolites of molds and have adverse effects on humans, animals, and crops. those can cause illnesses and economic losses. aflatoxin m1 (afm1) is one of the mycotoxins produced from the hydroxylated metabolite of aflatoxin b1 (afb1). it can be found in milk or milk products obtained from livestock that have ingested contaminated feed. in this paper, recent studies were...

Aegle marmelos leaves have been used for treatment of various liver aliments from time immemorial. Pre-treatment of A.marmelos leaves crude powder at its therapeutic dose (1000 mg/kg body weight) was carried out to determine the efficacy on aflatoxin B1 (AFB1) (1 mg/kg body weight) induced liver toxicity in rats. The crude powder of A.marmelos was found to be a potent inducer of the phase II de...

Aflatoxins (AFs) are highly toxic secondary metabolites produced by several Aspergillus, Penicillium and Rhizopus species in crops and plant products, of which aflatoxin B1 (AFB1) is the most representative (Goldblatt, 1969). If AFB1 is present in the feed of lactating animals, they will excrete aflatoxin M1 (AFM1) into their milk (Allcroft and Carnaghan, 1963; Cupid et al., 2004; Battacone et ...

The diterpenes cafestol and kahweol (C&K) have been identified in animal models as two potentially chemoprotective agents present in green and roasted coffee beans. It has been postulated that these compounds may act as blocking agents by producing a co-ordinated modulation of multiple enzymes involved in carcinogen detoxification. In this study, we investigated the effects of C&K against the c...

Aflatoxin B1 (AFB1) is a highly toxic compound (LD50 = 1-50 mg/kg) for most animal species, although it is extremely toxic (LD50 < 1 mg/kg) for some highly susceptible species such as pigs, dogs, cats, rainbow trouts, and ducklings. The toxic effects of AFB1 are both dose and time dependent and two distinct forms of aflatoxicosis, namely acute and chronic, can be distinguished depending on the ...

BACKGROUND Aflatoxins are known contaminants of foods. High dose exposure, particularly to Aflatoxin B1 (AFB1) may cause acute aflatoxicosis. Outbreaks have been reported in developing nations but are virtually un-documented in the developed world. CASE REPORT A 28 year old, healthy male presented with nausea, vomiting and abdominal pain. The patient deteriorated rapidly to a state of agitati...

To evaluate the role of aflatoxin B1 (AFB1) exposure on risk of hepatocellular carcinoma (HCC), a case-control study nested within a community-based cohort was conducted. Baseline blood and urine samples were used to determine the level of AFB1-albumin adducts and urinary AFB1 metabolites. Conditional logistic regression analysis was used to calculate odds ratios (OR) and 95% confidence interva...