Two useful [14C]marker proteins--[14C]human serum albumin (4.6S) and [14C]glucose oxidase (7.9S)--can be simply prepared. Both may be used as molecular-mass standards in polyacrylamide gel electrophoresis as sucrose density-gradient centrifugation. The utility of these markers for estrogen receptor studies was investigated under a variety of conditions, to ensure that they do not interfere with...

background: protein aggregation is one of the important, common and troubling problems in biotechnology, pharmaceutical industries and amyloid-related disorders. methods: in the present study, the inhibitory effects of some carbohydrates (alginate, β-cyclodextrin and trehalose) on the formation of nano-globular aggregates from normal (hsa) and glycated (ghsa) human serum albumin were studied; w...

background: progress in the field of biology and biochemistry has led to the discovery of numerous bioactive peptides and proteins in the last few decades. delivery of therapeutic proteins/peptides has received a considerable amount of attention in recent years. methods: in this study, a two-step desolvation method was used to produce biodegradable hydrophilic gelatin nanoparticles (gnp) as a d...

the aim of the present work was to prepare and characterize biopolymer nanocarriers and evaluate their suitability in possible oral delivery of insulin. the egg albumin biopolymer was used to prepare nanoparticles which were further characterized by fourier transformed infrared spectroscopy (ftir), transmission electron microscopy (tem), scanning electron microscopy (sem), zeta potential, dynam...

Introduction The relaxivity of an MR contrast agent (CA) parameterizes how efficiently a CA alters neighboring water proton longitudinal (T1) and transverse (T2) relaxation times. A priori knowledge of a CA relaxivity can be used in conjunction with preand post-contrast T1-weighted scans and native T1 values to determine the concentration of CA in a sample. CA relaxivity in solution can be read...

45 46 This study describes a high-throughput fluorescence dilution technique to measure the 47 albumin reflection coefficient (σAlb) of isolated glomeruli. Rats were injected with fluorescein 48 isothiocyanate–dextran 250 (75 mg/kg) and the glomeruli were isolated in a 6% bovine serum 49 albumin solution. Changes in the fluorescence of the glomerulus due to water influx in response 50 to an imp...

serum albumin is the most aboundant protein in blood plasma. its two major roles aremaintaining osmotic pressure and depositing and transporting compounds. in this paper,albumin-methanol solution simulation is carried out by three techniques including montecarlo (mc), molecular dynamic (md) and langevin dynamic (ld) simulations. byinvestigating energy changes by time and temperature (between 27...

Momentum is a simple and widely used trick which allows gradient-based optimizers to pick up speed in low curvature directions. Its performance depends crucially on a damping coefficient β. Large β values can potentially deliver much larger speedups, but are prone to oscillations and instability; hence one typically resorts to small values such as 0.5 or 0.9. We propose Aggregated Momentum (Agg...

background : since albumin imposes a relatively high cost to a healthcare system, drug use evaluation for this drug is much more important. this study wants to evaluate pattern of albumin use in a large university affiliated hospital in tehran, iran. methods : a concurrent, cross-sectional study was performed in “shaheed rajaei” cardiovascular, medical and research center. all inpatient adults ...