Calf pancreas microsomes incorporated radioactive mannose from GDP-D-[14C]mannose into products precipitated by 6% trichloroacetic acid-O.5 % phosphotungstic acid. This incorporation was maximally stimulated by 10 mM Mn2+ ions and showed a broad pH optimum of 6.0 to 6.7 in ‘I ris-maleate buffer. Between 70 and 90% of the radioactivity was found in products soluble in chloroform-methanol (2 : 1,...

This study investigated migration and transformation mechanism of P in waste activated sludge (WAS) during anaerobic fermentation (AF) process the subsequent hydrothermal conversion (HTC) process. Control pH AF processes was found to be significant, whereby use acidic (pH = 5.5) or alkaline conditions 9.5) facilitated release either apatite phosphorus (AP) non-apatite inorganic (NAIP) organic p...

90Sr has a long half-life, strong radioactivity, and high mobility. The removal of radioactive strontium from the water environment is great significance to human safety sustainable development nuclear energy. In this study, two-dimensional rare earth phosphate K3Nd(PO4)2 efficiently captured Sr2+ ions in aqueous solutions. At room temperature, adsorption isotherm, kinetics, pH dependence exper...

Large amounts of septic tank sludges from sanitation facilities are either landfilled or illegally dumped into the natural environment, leading to environmental pollution and waste resources. This issue calls for advanced methods recycle such as sustainable adsorbents phosphorus, e.g., in agriculture, context circular economy. Here, we hypothesized that alkaline sludge biochar could be an effic...

A sensitivity analysis was performed on a sediment-phosphate transport model by determining the phosphate sorption characteristics of separated size fractions and prepared size distributions of fine-grained ( < 64 jum) river sediment typically transported in two southern Ontario streams. The Langmuir equation was used to quantify phosphate release from the sediment. Results of the study indicat...

Wastewater Treatment Plants that make use of anaerobic processes for sludge digestion often have problems with phosphates and ammonia nitrogen “overloads” returned to biological sewage treatment process together with effluents coming from dewatering of digestion sludge. The high concentrations of phosphates and ammonium nitrogen in supernatant constitute 10 – 20 % of entire load brought to biol...

1. Lysosomal acid RNase was partially purified from a rat liver homogenate and a lysosomal fraction. This RNase, of optimum pH 5 to 6, cleaved RNA to form nucleoside 2’,3’cyclic phosphates, which were hydrolyzed to nucleoside 3’-monophosphates. This enzyme was heat-labile and had a molecular weight of about 24,000 to 28,000, as estimated by gel filtration with Bio-Gel P-60. This lysosomal acid ...

Metabolite levels in kernels of selected starch-deficient mutants of maize (Zea mays L.) were investigated to gain insight into partitioning of carbohydrate metabolism during kernel development. Several free sugars, hexose phosphates, triose phosphates, fructose-2,6-bisphosphate, and pyrophosphate were measured in normal, shrunken, shrunken-2, amylose extender dull waxy, and brittle genotypes, ...

Phosphates along the DNA function as chemical energy frequently used by nucleases to drive their enzymatic reactions. Exonuclease functions as a machine that converts chemical energy of the phosphodiester-chain into mechanical work. However, the roles of phosphates during exonuclease activities are unknown. We employed λ exonuclease as a model system and investigated the roles of phosphates dur...