Wheat (Triticum aestivum) RNA was used to program synthesis of the alpha-amylase protein by Xenopus laevis oocytes. A 41,500-dalton protein was made which was identified as alpha-amylase by immunoprecipitation with rabbit anti-alpha-amylase antiserum raised against the purified wheat protein and by its co-migration with authentic alpha-amylase on sodium dodecyl sulfate polyacrylamide gels. Synt...

We have isolated cDNA clones from rat C6 glioma cells coding for several guanine nucleotide-binding regulatory protein (G protein) alpha subunits (G alpha). The cDNA clones were then used to isolate human chromosomal genes. Among human genomic clones isolated by cross-hybridization with the rat cDNA for the alpha subunit of the inhibitory G protein Gi2, termed Gi2 alpha, a clone designated lamb...

responses of plants to salinity stress and development of salt tolerance are extremely complex and various mechanisms appear to be involved. we employed a proteomic approach to study the mechanism of plant responses to salinity in a sensitive cultivar of barley (527 line). three-week-old seedlings were treated with 300 mm nacl for 3 weeks. total proteins of fourth leaf were extracted and separa...

The purpose of this study was to determine the role of streptococcal M protein in naturally-occurring CD4+CD25+ regulatory T cells (nTregs) function and development in rheumatic heart disease in Iraqi patients. Streptococcus pyogenes was isolated for subsequent M protein extraction. Also, peripheral blood nTregs and CD4+ T cells were isolated by using Magnetic Cell Separation System. Tissue cul...

Antibiotic-resistance genes carried by coliforms in drinking water is a concerning issue for public health Bangladesh. This research was out to identify and understand the importance of int1 gene spread resistance bacterial antibiotics through consumption contaminated water. A total 31 samples were collected from restaurants ( n = 18), center 9), residences 4) located Chattogram City, The isola...

Parkinson's disease (PD) is the second most common neurodegenerative disorder worldwide, yet there no disease-modifying therapy up to this date. The biological complexity underlying PD hampers investigation of principal contributors its pathogenesis. In context, mechanistic models grounded in molecular-level knowledge provide virtual labs uncover primary events triggering onset and progression ...

Wingless (wg) is a segment-polarity gene in Drosophila which is related to the murine proto-oncogene int1. In Drosophila embryos, wg transcription defines part of each parasegment. In situ hybridization shows that wg is also expressed in the imaginal discs which give rise to the adult during metamorphosis. Transcripts are localized in the apical cytoplasm of disc cells, and accumulate in differ...

the automatic assignment of protein secondary structure from three dimensional coordinates is an essentialstep in the characterization of protein structure.although, the recognition of secondary structures suchas alpha-helices and beta-sheets seem straightforward, but there are many different definitions, eachregarding different criteria. we have developed a newalgorithm for protein helix assig...