Background: This study was set up to evaluate the effect of ovarian tissue slow freezing on in vitro growth and pattern of maturation genes expression in mouse preantral follicles encapsulated within alginate hydrogel. Materials and Methods: Ovaries of 12-14 days old female NMRI mice were randomly allocated into control and slow freezing groups. In slow freezing group, ovaries were equilibrated...

Cotton plays an important role in China’s agricultural production structure and international trade; therefore, China has implemented a variety of cotton subsidy policies. Since joined the WTO 2001, rules have become substantive constraints on its policy. Therefore, order to prevent appeal cases subsidy, this article, we investigate current situation optimization countermeasures with respect su...

A revision of the genus Helius Lepeletier & Serville, 1828 (Diptera: Limoniidae) from Baltic amber (Eocene) is presented. Redescriptions of 5 species, Helius formosus Krzemiński, 1993, Helius linus Podenas, 2002, Helius minutus (Loew, 1850), Helius mutus Podenas, 2002, Helius pulcher (Loew, 1850) of this genus from Baltic amber are given and documented by photographs and drawings. Four new spec...

We investigate two topics regarding solar mass FGK-type stars, the lithium-rotation connection (LRC), and existence of ‘lithium desert’. determine minimum critical rotation velocity ( v sin i ), related with LRC separating slow from rapid stellar rotators, to be 5 km s −1 . This value also splits different properties. For first time we explore behaviour for some associations ages between 45 Myr...

The original amber has a special esthetic value and for that is more and more commercialized on market as jewelry, articles for gifts, souvenirs, art pieces and décor objects. The amber quality is the result of its aspect (especialyl natural inclusions), shape, color, gloss, dimensions, processing methods and others. The modern technologies have permitted to fake the amber with some materials. ...

The precise engineering of proteins in bacteria via the amber codon has been hampered by the poor incorporation of unnatural amino acid (UAA). Here we explored the amber assignment as a sense codon for UAA by CRISPRi targeting release factor 1 (RF1). Scanning of RF1 gene with sgRNAs identified target loci that differentiate RF1 repressions. Quantitation of RF1 repressions versus UAA incorporati...

Multiple variants of the AMBER all-atom force field were quantitatively evaluated with respect to their ability to accurately characterize helix-coil equilibria in explicit solvent simulations. Using a global distributed computing network, absolute conformational convergence was achieved for large ensembles of the capped A(21) and F(s) helical peptides. Further assessment of these AMBER variant...