We reviewed the current methodologies used for human DNA quantitation in forensic and ancient DNA studies, including sensitive hybridization methods based on the detection of nuclear alpha-satellite repetitive DNA regions or more recently developed fluorogenic real-time polymerase chain reaction (PCR) designs for the detection of both nuclear and mitochondrial DNA regions. Special emphasis has ...

We reviewed the current methodologies used for human DNA quantitation in forensic and ancient DNA studies, including sensitive hybridization methods based on the detection of nuclear alpha-satellite repetitive DNA regions or more recently developed fluorogenic real-time polymerase chain reaction (PCR) designs for the detection of both nuclear andmitochondrial DNA regions. Special emphasis has b...

Although molecular dating of cladogenetic events is possible, no molecular method has been described to date the acquisition of various tissues. Taking into account the specificity of the major protein in enamel in formation (amelogenin), we were able to develop such a method for enamel. Indeed, because the amelogenin protein is exclusively involved in enamel formation and mineralization and be...

Mutations in the human enamelin gene cause autosomal dominant hypoplastic amelogenesis imperfecta in which the affected enamel is thin or absent. Study of enamelin knockout NLS-lacZ knockin mice revealed that mineralization along the distal membrane of ameloblast is deficient, resulting in no true enamel formation. To determine the function of enamelin during enamel formation, we characterized ...

and Emdogain® to Plastic by Enzyme-linked Immunosorbent Assays (ELISA) To assess whether recombinant amelogenin and Emdogain® used in the cell attachment experiments indeed coupled to plate surfaces, we coated a two-fold serial concentration range (1.5 g–1.5 and 0 ng/well) of each protein in duplicate on Dyclone 2HB 96-microwell plates overnight at 4°C in MAM, imitating the conditions used in t...

The aim of this paper was to determine whether the interaction between IGF, IGFBP, and VN modulates the functions of porcine EOE cells. Enamel organs from 6-month-old porcine third molars were dissociated into single epithelial cells and subcultured on culture dishes pretreated with VN, IGF-I, and IGFBP-3 (IGF-IGFBP-VN complex). The subcultured EOE cells retained their capacity for ameloblast-r...

Duchenne muscular dystrophy (DMD) is an X-linked recessive genetic disorder with mutational heterogeneity. The scarcity of DNA from single cells in preimplantation genetic diagnosis (PGD) for DMD limits comprehensive genetic testing. Multiple displacement amplification (MDA) is reported to generate large amounts of template and give the most complete coverage and unbiased amplification to date....