Serpersu and Tsong (Sepersu, E. H., and Tsong, T. Y. (1983) J. Membr. Biol. 74, 191-201; (1984) J. Biol. Chem. 259, 7155-7162) reported activation of a K+ pumping mode of (Na,K)-ATPase by an oscillating electric field (20 V/cm, 1.0 kHz). Their attempts to activate Na+ pumping at the same frequency were unsuccessful. We report here activation of a Na+ pumping mode with an oscillating electric fi...

Our aim was to investigate the effects of one year recombinant human growth hormone (rhGH) therapy on the regulation by insulin of gene expression in muscle and adipose tissue in adults with secondary GH deficiency (GHD). Six GHD subjects without upper-body obesity were submitted to a 3-h euglycemic hyperinsulinemic clamp before and after one year of rhGH therapy. Muscle and abdominal subcutane...

Nitrous oxide (N(2)O) emission from soils is a major contributor to the atmospheric loading of this potent greenhouse gas. It is thought that autotrophic ammonia oxidizing bacteria (AOB) are a significant source of soil-derived N(2)O and a denitrification pathway (i.e. reduction of NO(2) (-) to NO and N(2)O), so-called nitrifier denitrification, has been demonstrated as a N(2)O production mecha...

The appetite suppressant dexfenfluramine, which inhibits neuronal 5-HT uptake and elevates plasma 5-HT levels, has been associated with an increase in the relative risk of developing primary pulmonary hypertension. 5-HT is a mitogen for pulmonary artery smooth muscle cells (PA-SMCs), an effect that depends upon activity of the 5-HT transporter (5-HTT). To investigate the relationship between de...

The distribution and activity of the bulk picoplankton community and, using microautoradiography combined with catalysed reported deposition fluorescence in situ hybridization (MICRO-CARD-FISH), of the major prokaryotic groups (Bacteria, marine Crenarchaeota Group I and marine Euryarchaeota Group II) were determined in the water masses of the subtropical North Atlantic. The bacterial contributi...

The ability to reverse the net direction of gill ion transport in response to a salinity change is critical for euryhaline teleosts and involves a complex cellular and molecular remodelling of the gill epithelium. The present study aimed to clarify the cellular localisation and exact quantitative inter-relationship of Na(+),K(+)-ATPase alpha- and beta-subunit transcripts in Atlantic salmon gill...

BACKGROUND The T helper cell type 2-associated cytokine interleukin (IL)-10 has a variety of immunomodulatory properties. However, the effects of the cytokine on viral myocarditis remain unclear. METHODS AND RESULTS We studied the effects of recombinant human IL-10 (rhIL-10) fully active on mouse cells in a murine experimental model of acute viral myocarditis caused by the encephalomyocarditi...

Biological and biomedical applications of accelerator mass spectrometry (AMS) use isotope ratio mass spectrometry to quantify minute amounts of long-lived radioisotopes such as (14)C. AMS target preparation involves first the oxidation of carbon (in sample of interest) to CO 2 and second the reduction of CO 2 to filamentous, fluffy, fuzzy, or firm graphite-like substances that coat a -400-mesh ...

background: one of the most important ways of pathologic and non-pathologic parasites transmission is through eating raw vegetables. iranian people believe that eating raw vegetables is an important way to get some essential vitamins and minerals. objectives: according to the high prevalence of pathologic parasites and also exporting different vegetables to the other provinces such as tehran, w...