نتایج جستجو برای: amperometry

تعداد نتایج: 456  

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 2003
Liang-Wei Gong Ismail Hafez Guillermo Alvarez de Toledo Manfred Lindau

The number of transmitter molecules released in a quantal event can be regulated, and recent studies suggest that the modulation of quantal size is associated with corresponding changes in vesicle volume (Colliver et al., 2000; Pothos et al., 2002). If so, this could occur either by distension of the vesicle membrane or by incorporation and removal of vesicle membrane. We performed simultaneous...

2005
Fiachra B. Bolger John P. Lowry

In this communication we review selected experiments involving the use of carbon paste electrodes (CPEs) to monitor and measure brain tissue O2 levels in awake freely-moving animals. Simultaneous measurements of rCBF were performed using the H2 clearance technique. Voltammetric techniques used include both differential pulse (O2) and constant potential amperometry (rCBF). Mild hypoxia and hyper...

2011
Arun Anantharam Mary A. Bittner Rachel L. Aikman Edward L. Stuenkel Sandra L. Schmid Daniel Axelrod Ronald W. Holz

Dynamin is a master regulator of membrane fission in endocytosis. However, a function for dynamin immediately upon fusion has also been suspected from a variety of experiments that measured release of granule contents. The role of dynamin guanosine triphosphate hydrolase (GTPase) activity in controlling fusion pore expansion and postfusion granule membrane topology was investigated using polari...

2008
Lauren E. Frank Angela D. Caldera-Siu Emmanuel N. Pothos

The ability to create primary cell cultures of dopamine neurons allows for the study of the presynaptic characteristics of dopamine neurons in isolation from systemic input from elsewhere in the brain. In our lab, we use these neurons to assess dopamine release kinetics using carbon fiber amperometry, as well as expression levels of dopamine related genes and proteins using quantitative PCR and...

Journal: :FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012
Jésica Díaz-Vera Marcial Camacho José David Machado Natalia Domínguez Mónica S Montesinos Juan R Hernández-Fernaud Rafael Luján Ricardo Borges

Chromogranins are the main soluble proteins in the large dense core secretory vesicles (LDCVs) found in aminergic neurons and chromaffin cells. We recently demonstrated that chromogranins A and B each regulate the concentration of adrenaline in chromaffin granules and its exocytosis. Here we have further studied the role played by these proteins by generating mice lacking both chromogranins. Su...

2016
Selvakumar Palanisamy Balamurugan Thirumalraj Shen-Ming Chen Yi-Ting Wang Vijayalakshmi Velusamy Sayee Kannan Ramaraj

We report a novel and sensitive amperometric sensor for chlorpromazine (CPZ) based on reduced graphene oxide (RGO) and polydopamine (PDA) composite modified glassy carbon electrode. The RGO@PDA composite was prepared by electrochemical reduction of graphene oxide (GO) with PDA. The RGO@PDA composite modified electrode shows an excellent electro-oxidation behavior to CPZ when compared with other...

2012
Yang Wang Guojun Yao Jie Tang Chun Yang Qin Xu Xiaoya Hu

The potential capabilities and analytical performance of lab-on-valve (LOV) manifold as a front end to amperometry have been explored for the on-line determination of morin. Meanwhile, the electrochemical behaviors of morin were investigated based on polyvinylpyrrolidone- (PVP-) doped carbon paste electrode (CPE), which found that PVP can significantly improve its oxidation peak current. The ex...

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