Starch, total amylolytic and phosphorylase activities were determined in lentil cotyledons during the first days of germination. Several independent criteria show that the amylolytic activity is due mainly to an amylase of the alpha type. Starch is degraded slowly in the first days; during this time, alpha- and beta-amylase activity are very low, while phosphorylase increases and reach a peak o...

Four hundred and forty 1-day-old Arbor Acre broilers were fed commercial starter diets with 0, 250, 750 and 2,250 mg/kg of an alpha-amylase preparation from 1 to 21 days of age to investigate the effects of an exogenous enzyme on growth performance, activities of digestive enzymes in the pancreas and anterior intestinal contents and pancreatic amylase mRNA expression. Body weight gain (BWG) and...

Several investigated bank vole populations are polymorphis for the number of salivary amylase loci, and individual chromosomes may carry one, two or three linked amylase structural genes. In the present study, we have used bank vole stocks homozygous for different chromosomes to investigate the relationship between amylase production and gene number. By measuring the amylase activity in parotid...

Purification of alpha-amylase from the cultivation supernatant of recombinant Bacillus subtilis by high-speed counter-current chromatography (HSCCC) in polyethylene glycol (PEG) 4000-inorganic salt aqueous polymer two-phase systems was studied. The effects of sodium chloride concentration on the partition coefficients of alpha-amylase and total protein were respectively tested in PEG4000-phosph...

Experiments were carried out in vivo on rats and in vitro on tubular brush border vesicles in order to study the renal mechanisms of the elimination of pancreatic lipase and amylase from the circulation. Highly purified 125I-labelled homologous lipase, amylase or 125I-labelled di-iodo-tyrosine was injected intravenously in a single dose. The sieving coefficients of lipase and amylase were found...

Aim: To compare the amylase activity in the cervical mucus and serum during estrus in normal and repeat breeder cattle and find out importance in fertility. Materials and methods: Twenty cyclic crossbred cattle were divided into two groups: normal (n =10) and repeat breeder (n =10). Animals exhibiting spontaneous estrus, were artificially inseminated (AI) twice, as per AM-PM schedule along with...

HE CHOICE OF RAPID METHODS for estimation of serum amylase activity is limited (1). Such methods depend upon the change in color of starch with iodine brought about by splitting of the starch by the enzyme. it is often difficult to establish an end point visually by this reaction in partially digested starch solutions, especially by those whose experience with the method is limited. Furthermore...