In this study, changes in angiotensin-I converting enzyme (ACE) inhibitory, α-amylase inhibitory and antioxidant activities, total phenolic content (TPC), monomeric anthocyanin (TMAC) of ultrasonic extracts from pulp seed Ziziphus jujuba were investigated during vitro digestion. Bioaccessible fractions phenolics calculated as 23.24±4.46% 9.43±0.24%, respectively. Moreover, bioaccessibility for ...

The heterotrimeric G-protein Gq/11 was identified on pancreatic acinar zymogen granules and its function in calcium-regulated exocytosis was examined. Western blotting showed alphaq/11, but not alphas or alphao, to be localized to the zymogen granule membrane along with G-protein beta-subunit; all three alpha subunits were present in a plasma membrane fraction and the alphaq/11 signal was 30-fo...

Amylases are one of the most important enzymes in present-day biotechnology. The objective of this study was to isolate and identify a potential thermophilic amylase producing bacterial strain from hot spring of Bakreswar village, Suri, West Bengal (India). Among the few isolated amylolytic strains on starch agar medium, the strain IB-A showed best amylase activity. Phylogenetic analysis based ...

Objective(s):Diabetes mellitus, a carbohydrate metabolic disorder, occurs due to absolute or relative deficiency of insulin. Current treatment strategies involve either preventing or delaying the intestinal absorption of glucose to lower the levels of postprandial hyperglycemia (PPHG). Herbal remedies have been since ancient times for treating diabetes mellitus. Therefore, identifying novel phy...

Corn tassel (Zea may L.) is rich in phenolic compounds including flavonoids and anthocyanins. The aim of this study to consider the results contents phenolics (TPC), (TFC), flavonol (TF), anthocyanins (TAC), alfa-amylase inhibitory activity, antioxidant activity FRAP metal chelating capabilities (MCC) as potential Antiviral anti-Rheumatoid arthritis. Significantly high levels capacity, total in...

This study aimed to evaluate and correlate phytochemical content, antioxidant activity alpha-amylase inhibitory in aqueous acetone extract fractions derived from it (ethyl acetate fraction, watersoluble methanol fraction fraction) which are obtained Ampelocissus martini Planch. root. Ethyl had the highest total phenolic content proanthocyanidin respectively. 2,2-diphenyl-1-picrylhydrazyl, 2,2'-...

Abstract Objective To provide the scientific basis for utility of rhizome Trillium govanianum as nutraceutical supplements in managing physiological glycemic levels. Methods The vitro enzyme inhibitory activity extract, fractions, and isolated steroidal saponins from part T. was carried out against ?-amylase, ?-glucosidase, dipeptidyl peptidase IV. molecular interactions, binding score, pharmac...

The side effects associated with the usage of synthetic antidiabetic drugs make it imperative to search for alternative drugs from medicinal plants. Therefore, this study was aimed at evaluating the α-amylase and α-glucosidase inhibitory potential of Calotropis procera leaf, as well as its possible mode of inhibiting these enzymes. Acetone, aqueous and ethanolic extracts of C. procera leaf was ...

Alpha-amylase inhibitor protein, which inhibits the activity of insect (Callosobruchus analis) α-amylase, was characterized from V. sublobata. The molecular weight of purified inhibitor protein was 14 kDa by SDS-PAGE. The inhibitor is non-glycosylated protein and its N-terminal sequence is similar (A P S P V...) to Phaseolus vulgaris α-AI-1. Its pI value is 6.0 and largely localised in cotyledo...

An alpha-amylase inhibitor was prepared from cranberry bean (Phaseolus vulgaris). The alpha-amylase inhibitor was composed of three different subunits not linked by disulfide bridges and only one of them contained carbohydrate. Although the inhibitor was stable at pH 3 to 7, it was heat labile at pH 3 and 5. Chemical modification of the amino groups and the guanido groups in cranberry bean alph...