Guinea pig reticulocytes were pulse-labelled with 59Fe bound to transferrin. Haemolysates prepared from these reticulocytes were subjected to rapid (NH1)2SO1 precipitation and then chromatography on an anion-exchange resin. ATP-bound 59Fe was the dominant species in the reticulocyte cytosol; 2,3-bisphosphoglycerate and GTP iron complexes were not detected despite the fact that these were stable...

When a series of patients' urine samples supplemented in vitro with chlorpromazine or imipramine was assayed with the Forrest qualitative assays, we observed an occasional false-negative result, which we found was attributable to interference by ascorbic acid. It interferes with the reagent, not with the analytes, in both assays. We easily eliminated this interference with the phenothiazine tes...

I describe an improved procedure for estimating thyroxine in dried blood on 0.32-cm filter paper discs, by radioimmunoassay. A weak anion-exchange resin is used to separate free and antibody-bound radiothyroxine and, by measuring the resin-bound 125I-labeled thyroxine rather than the supernatant radioactivity, a tedious and cumbersome step is eliminated. The unique properties of the resin permi...

We describe a "high-performance" liquid-chromatographic procedure for measuring 3-methoxy-4-hydroxymandelic acid (vanillylmandelic acid) in urine. The compound is quantitatively extracted from urine by use of an automated system, including an anion-exchange resin. After elution from the resin and concentration by air drying, the urine extract is chromatographed on an octyl silica column, with u...

The uptakes of cycloalkanes (C5 - C8) and bicyclic aromatic compounds (naphthalene, biphenyl, and azulene) by both strong acid cation (Dowex 50W-X4 and X8) and strong base anion (Dowex 1-X4 and X8) exchange resins have been studied in aqueous solutions at 25 degrees C; their distribution constants (K) have been determined. The relationship between the octanol-water partition coefficient and the...

Thorough removal of silicic acid from treated water, as a water pretreatment procedure, is essential for the prevention silica deposits on anion exchange resins and reverse osmosis membranes. In this study, thorough was achieved using Tiron (1,2-dihydroxy-3,5-benzendisulfonate) combined resin. column experiment, it confirmed that could be removed below detection limit molybdenum-blue methods. T...