Human adenylosuccinate synthetase (IMP:aspartate ligase (GDP) EC 6.3.4.4) has been purified 1 IO-fold from placenta. The partially purified preparation was stable and free of nucleotidase activity, permitting study of substrate kinetics and regulatory properties. The Michaelis constants for the substrates, IMP, GTP, and aspartate were 37 PM, 31 PM, and 0.95 m&x, respectively. Based on studies o...

BACKGROUND Studies on health-related quality of life (HRQoL) of patients awaiting pacemaker (PM) implantation are scarce, or executed in specific patient subgroups (regarding age or specific cardiac rhythm disorders). The purpose of this study was to systematically assess the HRQoL in a large unselected cohort of patients with a conventional indication for PM therapy. METHODS Pre-PM implantat...

Vascular endothelial growth factor (VEGF)/vascular permeability factor (VPF), an endothelial cell (EC)-specific mitogen, stimulates angiogenesis in vivo, particularly in ischemic regions. VEGF/VPF expression by cells of hypoxic tissues coincides with expression of its two receptors, KDR and flt-1, by ECs in the same tissues. We investigated whether hypoxia or hypoxia-dependent conditions operat...

The renal UT-A urea transporters UT-A1, UT-A2, and UT-A3 are known to play an important role in the urinary concentrating mechanism. The control of the cellular localization of UT-A transporters is therefore vital to overall renal function. In the present study, we have investigated the effect of ubiquitination on UT-A plasma membrane expression in Madin-Darby canine kidney (MDCK) cell lines ex...

UNLABELLED This self-directed learning module highlights physical medicine and rehabilitation (PM and R) interventions for age-related physiologic changes. It is part of the study guide on geriatric rehabilitation in the Self-Directed Physiatric Education Program for practitioners and trainees in PM and R and geriatric medicine. This article specifically focuses on PM and R interventions (inclu...

Vascular endothelial growth factor (VEGF)/vascular permeability factor (VPF), an endothelial cell (EC)–specific mitogen, stimulates angiogenesis in vivo, particularly in ischemic regions. VEGF/VPF expression by cells of hypoxic tissues coincides with expression of its two receptors, KDR and flt-1 , by ECs in the same tissues. We investigated whether hypoxia or hypoxia-dependent conditions opera...

Over the last few years X-ray observations of broad-line radio galaxies (BLRGs) by ASCA, RXTE and BeppoSAX have shown that these objects seem to exhibit weaker X-ray reflection features (such as the iron Kα line) than radio-quiet Seyferts. This has lead to speculation that the optically-thick accretion disc in radio-loud active galactic nuclei (AGN) may be truncated to an optically-thin flow in...

We present the results of XMM-Newton observations of four high-z quasars, two radio-loud and two radio-quiet. One of the radio-loud objects, PKS 2126−158, clearly shows absorption in excess of the galactic value as claimed before from ASCA and ROSAT observations. For PKS 2149−306 the evidence for excess absorption is only marginal in contrast to previous results. The location of the absorber in...

We present the analysis and interpretation of a sample of eight ASCA observations of Broad Absorption Line Quasi-Stellar Objects (BALQSOs). This is the first moderate-sized sample of sensitive BALQSO observations above 2 keV, and the BALQSOs in our sample are among the optically brightest known (B = 14.5–18.5). Despite the ability of 2–10 keV X-rays to penetrate large column densities, we find ...