The majority of cord blood transplantations (CBTs) have human leukocyte antigen (HLA) disparities. We investigated the impact that patients' pretransplantation anti-HLA antibodies have on the outcome of CBTs. Testing for anti-HLA antibody and its specificity was performed retrospectively at the Japanese Red Cross Tokyo Blood Center with sensitive solid-phase antibody detection assays. Among 386...

OBJECTIVE The aim of this study was to assess the antibody response to combined passive-active immunization versus active immunization against hepatitis B in 71 patients with acute leukemia with negative hepatitis B virus serology at presentation. METHODS The first group (n=28) received a double dose of hepatitis B vaccine at 0, 1, 2 and 6 months and immunoglobin (HBIG) at 0 and 1 month concu...

Background: In spite of available, and sensitive screening assay for detection of hepatitis B virus surface antigen (HBsAg), occasional cases of post-transfusion hepatitis B virus infection are still observed. The aim of the present study was to assess the prevalence of positive anti hepatitis B core (anti-HBc) and presence of HBV-DNA in serum sample of healthy blood donors negative for both HB...

background: hepatitis- b infection is one of the infectious diseases that transmits via blood transfiosion, blood product, injection, mother to fetus and sexual contact. protective anti hepatitis-b titer are observed in more than 90% of healthy children. the aim of this study was to evaluate seroconversion rates in children who received routine vaccination program. methods: a cross sectional st...

The enzyme-linked immunospecific antibody test was performed in standard test tubes and microtiter plates to meausre high-titer antibody against Klebsiella capsular polysaccharide. Initial studies were conducted with rabbit sera; other studies were conducted with the serum of a patient infected with type 9 Klebsiella. Both immunized rabbits and an infected patient disclosed high titers of anti...

BACKGROUND Different studies have demonstrated that a small proportion of healthy individuals receiving the hepatitis B (HB) vaccine do not produce protective levels of anti-HB antibody, a phenomenon which could be linked to certain human leukocyte antigen (HLA) class-II alleles or haplotypes. OBJECTIVES The present study was undertaken to determine the frequency of HLA class-II alleles in Ir...

Invariable region (IR)(6), an immunodominant conserved region of VlsE, the antigenic variation protein of Borrelia burgdorferi, is currently used for the serologic diagnosis of Lyme disease in humans and canines. A longitudinal assessment of anti-IR(6) antibody levels in B. burgdorferi-infected rhesus monkeys revealed that this level diminished sharply after antibiotic treatment (within 25 week...

To investigate the utility of cerebrospinal fluid (CSF) anti-feline coronavirus (FCoV) antibody test for diagnosis of feline infectious peritonitis (FIP), the antibody titers were tested in CSF and sera from 271 FIP-suspected neurological cats. CSF antibody was detected in 28 cats, which were divided into 2 groups; 15 with CSF titer of 1:80 or lower and 13 with CSF titer of 1:640 or higher. In ...

Autoimmune neutropenia (AIN) of early childhood is caused by autoantibodies against antigens on the neutrophil membrane. Human leukocyte (HLA) have previously been associated with AIN. This study investigated HLA-DRB1 and HLA-DQB1 alleles in 160 antibody positive patients compared 1000 controls. Increased risk was observed for DRB1*10, DRB1*14, DRB1*16 DQB1*05, lower DRB1*04, DRB1*13 DQB1*03. H...