Highly purified C'1 esterase of human serum is capable of inactivating isolated fourth component of human complement (beta(1E)-globulin). Inactivation is accompanied by changes in electrophoretic and ultracentrifugal properties of beta(1E)-globulin. If non-sensitized sheep erythrocytes are present during the action of C'1 esterase on beta(1E)-globulin, a complex is formed consisting of cells an...

Immune dysregulation is a mechanism contributing to ineffective hematopoiesis in a subset of myelodysplastic syndrome patients. We report the first US multicenter non-randomized, phase II trial examining the efficacy of rabbit(r)-anti-thymocyte globulin using 2.5 mg/kg/day administered daily for 4 doses. The primary end point was hematologic response; secondary end points included duration of r...

Erythrocytes from mink chronically infected with Aleutian disease virus (ADV) gave positive antiglobulin reactions with rabbit anti-mink immunoglobulin (Ig)G, anti-mink C3, and anti-mink serum, but did not react with anti-mink IgM. The strongest reaction was observed with anti-mink C3. Immunoelectrophoresis demonstrated that serum from rabbits injected with erythrocytes from ADV-infected mink g...

Studies of connective tissue are germane to the investigation of the rheumatic diseases, and many different technical procedures have been used in the study of the histogenesis, metabolism, and pattern of reaction of connective tissue to noxious influences. Recently, immuno-histochemical procedures based on the method evolved by Coons and his colleagues (Coons and Kaplan, 1950; Coons, Leduc, an...

In most rapid radioimmunoassay methods, absorbents are used that disturb the equilibrium of the ligand-antibody interaction; thus the separation process must be rigidly timed. We show here that polyethylene glycol (mol wt. 6000) abolishes this constant. We measured gamma-globulin precipitation by polyethylene glycol (150g/liter) in a thyroxine radioimmunoassay system involving use of a sheep an...

The fractional rates of catabolism of isotopically labeled mouse, human, bovine, and guinea pig gamma-globulins and human serum albumin were determined in mice and in guinea pigs whose serum gamma-globulin and serum albumin levels were elevated by immunization or by injections of exogenous serum proteins. These serum proteins were also followed in mice with different serum gamma-globulin levels...

The "inhibitor" demonstrable in extracts of the virus-induced rabbit papillomas is identical with the antiviral antibody found in the blood of hosts bearing the growths. The conditions in these latter are frequently favorable to its extravasation in considerable amount into them. Its significance and its influence upon the recovery of virus from the papillomas are discussed.

Anti-D immunoglobulin is now widely used to prevent Rh-negative mothers from being sensitized by Rh-positive fetal erythrocytes. The administration of anti-D following the transfusion of large amounts of Rh-positive erythrocytes into Rh-negative individuals has been less uniformly successful in preventing primary immunization (Bowman and Chown, 1968), and occasional mild (Woodrow et al, 1968) o...