There are increasing clinical (1-6), functional (7), radiological (4, 5, 6, 8), and histological (3, 9-14) evidences that interstitial pneumonitis with or without pulmonary fibrosis may develop in patients with underlying diseases which appear to be associated with circulating antigen-antibody complexes. The pathogenesis of these human lesions, however, has not been studied in experimental anim...

Abstract BACKGROUND: Diagnosis of Echinococcus granulosus in the definitive host particularly in dog is the significant complication in the endemic area. OBJECTIVES: The aim of this study is serological detection of E. granulosus in the infected dogs. METHODS: Dot-ELISA based on the copro-antigen and recombinant EPC1 antigen (rEPC1) for antibody detection was performed. Blood and fecal samples ...

Rabbit antibodies for Candida albicans, reacting in agglutination and fluorescent-antibody reactions, were present in both IgM and IgG protein fractions. The two types of immune globulins were separated from ammonium sulfate-precipitated gamma-globulin either by filtration through a column of Sephadex G-200 or by diethylaminoethyl column chromatography performed by stepwise elution with various...

There are increasing clinical (1-6), functional (7), radiological (4, 5, 6, 8), and histological (3, 9-14) evidences that interstitial pneumonitis with or without pulmonary fibrosis may develop in patients with underlying diseases which appear to be associated with circulating antigen-antibody complexes. The pathogenesis of these human lesions, however, has not been studied in experimental anim...

We report use of U-shaped biconically tapered optical fibers (BTOF) as probes for label-free immunoassays. The tapered regions of the sensors were functionalized by immobilization of immunoglobulin-G (Ig-G) and tested for detection of anti-IgG at concentrations of 50 ng/mL to 50 µg/mL. Antibody-antigen reaction creates a biological nanolayer modifying the waveguide structure leading to a change...

The objective of the paper is to develop a “lab-on-a-chip” device for early disease (pancreatic cancer) diagnosis by using ferromagnetic resonance (FMR). Magnetic microbeads, which are functionalized for target molecules (antigens), are immobilized by antigen-antibody reactions on the surface of a microwave circuit. These magnetic labels are detected inductively using FMR, which detects a singl...

From the results of numerous experiments, immunologists have inferred that when a foreign material, usually protein in nature and called an antigen, is injected into an animal, it induces the formation of substances called antibodies, which appear in the animal’s blood serum and which will react with the antigen injected (l-4). When this reaction results in the formation of a precipitate, it is...

Objective(s): We previously conducted an in silico research on the interactions between the ribosome display-selected single chain variable fragment (scFv) and factor H binding protein (fHbp) of Neisseria meningitidis. We found that heavy chain variable (VH) fragment of this scFv had considerable affinity to fHbp. These results led us to evaluate the ability of this sm...

The specificity of the recombinant Toxocara canis antigen developed for the immunodiagnosis of human toxocariasis was compared with that of the excretory-secretory antigen from T. canis second-stage larvae (TES) by enzyme-linked immunosorbent assay. A total of 153 human serum samples from patients infected with 20 different helminths, including 11 cases of toxocariasis, were examined. No false-...