The removal of excess free cholesterol from cells by HDL or its apolipoproteins is important for maintaining cellular cholesterol homeostasis. This process is most likely compromised in the atherosclerotic lesion because the development of atherosclerosis is associated with low HDL cholesterol. Multiple mechanisms for efflux of cell cholesterol exist. Efflux of free cholesterol via aqueous diff...

A common accuracy-based standardization program is indispensable for establishing reference intervals for the clinical use of apolipoproteins. The development and distribution of reference materials and quality-control materials that do not exhibit matrix effects between methods is essential to the standardization process. We examined the suitability of lyophilized material as a common referenc...

In 25 middle-aged infarction survivors and 76 corresponding controls, representative for a well-defined community, multivariate analysis was used to evaluate whether serum apolipoproteins were better discriminators of infarction survivors than serum lipids and other risk factors. Levels of serum cholesterol and triglycerides, alphalipoprotein cholesterol, apolipoproteins A-I, A-II, B, and D, as...

HDL cholesterol (HDL-C) efflux function may be a more robust biomarker of coronary artery disease risk than HDL-C. To study HDL function, apoB-containing lipoproteins are precipitated from serum. Whether apoB precipitation affects HDL subspecies composition and function has not been thoroughly investigated. We studied the effects of four common apoB precipitation methods [polyethylene glycol (P...

O September 21 and 22,1982 the Lipid Metabolism and Atherogenesis Branch of the National Heart, Lung, and Blood Institute sponsored a workshop on apolipoprotein quantification in Chevy Chase, Maryland. The purpose of the workshop was to bring together a large number of investigators with primary interests in epidemiology, genetics, and basic and clinical research to discuss recent advances in t...

Because lipoproteins containing apolipoprotein A (ApoA-I + ApoA-II) or apolipoprotein B (ApoB) seem to exert opposite effects as risk factors for coronary heart disease, we decided to determine the separability of these two major plasma lipoproteins by procedures originally designed to separate high-density from low- and very-low-density lipoproteins. The presumably ApoB-free lipoproteins isola...