conclusions optimal wc values for defining mets were 91.5 cm and 85.5 cm in men and women, respectively. the cut-off values recommended by world organizations are not appropriate for an adult population living in ahvaz city. results the cut-off points of wc, according to a maximum sum of sensitivity plus specificity for detecting at least two components of mets were found to be 91.5 cm and 85.5...

background: the objective of this study was to investigate the effect of vitrification and in vitro maturation on the mitochondrial distribution and atp content of oocytes. methods: the oocytes at germinal vesicle (gv) and metaphase ii (mii) stages were recovered from 6-8 week old nmri strain female mice. the oocytes were divided into vitrified and non-vitrified groups. vitrification was done b...

We develop a riboadenosine-substituted DNA probe-based bioluminescence method for real-time monitoring of exonuclease III (Exo III) activity and Exo III-assisted target recycling. This method employs the target-dependent ATP as the bioluminescence reporter without the involvement of any sophisticated luciferase manipulations, and the glow-type reaction kinetics facilitates the performance of im...

Many important cellular membrane fission reactions are driven by ESCRT pathways, which culminate in disassembly of ESCRT-III polymers by the AAA ATPase Vps4. We report a 4.3 Å resolution cryo-EM structure of the active Vps4 hexamer with its cofactor Vta1, ADP·BeFx, and an ESCRT-III substrate peptide. Four Vps4 subunits form a helix whose interfaces are consistent with ATP binding, is stabilized...

Quantitative effects of altering oxidative phosphorylation and respiration on the activity of the enzyme menadione reductase (NAD[P]H,:2-methyl-i,4-naphthoquinone oxidoreductase, E.C. 1.6.5.2), in stabilized polymorphonuclear neutrophil leucocytes of Amphiuma tridactylum, were studied by amplitude-contrast microscopy and microspectrophotometry. In a general way, the rate of enzymic activity was...

Previously, we showed that protein kinase B (Akt) activation increases intracellular ATP levels and decreases necrosis in renal proximal tubular cells (RPTC) injured by the nephrotoxicant S-(1, 2-dichlorovinyl)-l-cysteine (DCVC) (Shaik ZP, Fifer EK, Nowak G. Am J Physiol Renal Physiol 292: F292-F303, 2007). This study examined the role of Akt in improving mitochondrial function in DCVC-injured ...

Introduction To integrate and execute a proper preventive plan reduce the risk of non-communicable diseases (NCDs), policy makers need to have access both reliable data unique definition metabolic syndrome (MetS). This study was conducted on collected by cross-sectional studies WHO’s STEPwise approach surveillance NCD factors (STEPs) estimate national sub-national prevalence rates MetS in Iran ...