Our working hypothesis was that synthesis and release of LH, but not FSH, were solely dependent on LHRH. Twenty ovariectomized (OVX) ewes were randomly assigned to one of five treatments (n = 4 per group). Ewes were administered a low (10 micrograms/kg) or high (100 micrograms/kg) dose of LHRH antagonist (LHRH-Ant) at 24-h intervals for 3 or 6 days. Control ewes received vehicle (5% mannitol) a...

Objective- In the present study the role of essential oil of Salvia rhtidia (SR), in ameliorating renal ischemia reperfusion (IR) injuries is evaluated. Design- Experimental in vivo study. Animals- 48 male healthy Wistar rats between 250-300 grams. Procedures- The animals were randomly divided into eight groups of six rats. In ischemic groups (groups A-D), under general anesthesia b...

The aim of the present study was to examine the effects of scriptaid (SCR), a histone deacetylase inhibitor (HDACi), on the in vitro and in vivo development of Wuzhishan miniature pig somatic cell nuclear transfer (SCNT) embryos. Experiment 1 compared the in vitro developmental competence of nuclear transfer embryos treated with various concentrations of SCR for 24 h. Embryos treated with 250 n...

STUDY OBJECTIVE To determine the steady-state, extracellular, and intracellular pulmonary disposition of moxifloxacin (MXF), levofloxacin (LEVO), and azithromycin (AZI) relative to that of the plasma over a 24-h dosing interval. DESIGN Randomized, multicenter, open-label investigation. PATIENTS Forty-seven older adults (mean [+/- SD] age, 62 +/- 13 years) undergoing diagnostic bronchoscopy....

Materials and methods Experimental set-up CHO-K1 cells were cultivated in protein free TC-42 medium (TeutoCell, Bielefeld, Germany) in 250 ml baffled shake flasks. For the non-stationary experiment the cultures were inoculated at a start cell density of 2 × 10 cells/ml in a start volume of 120 ml. Four parallel cultivations were performed, two with 100% [U-C6] glucose and two with 100% [U-C5]gl...

We examined the effects of human interleukin 1 (IL-1) on the production of fibrinolytic components by cultured human vascular endothelium. Conditioned media collected from IL-1-treated (5 U/ml, 24 h) monolayers exhibited decreased tissue-type plasminogen activator (tPA) activity and increased plasminogen activator inhibitor (PAI) activity, as assessed by fibrin and reverse fibrin-autography. Qu...

An in vitro pharmacodynamic system has been successfully adapted to simulate in vivo antimicrobial pharmacokinetics under anaerobic conditions. This system was used to perform time-kill kinetic studies which were designed to compare the activity of temafloxacin to ciprofloxacin and cefotetan against two strains of Bacteroides fragilis (ATCC 25285 and ATCC 23745). All experiments were performed ...

Objective To evaluate the effects of an ultrasound-guided transverse abdominis plane (US-TAP) block used for postoperative pain relief by comparing the efficacy of two different volumes/concentrations of the local anaesthetic bupivacaine in patients undergoing laparoscopic cholecystectomies. Methods This randomized study enrolled patients undergoing laparoscopic cholecystectomies. They were ran...

OBJECTIVE This study aimed to a) compare the efficacy of metoclopramide and erythromycin in the treatment of feed intolerance in critical illness; and b) determine the effectiveness of "rescue" combination therapy in patients who fail monotherapy. DESIGN Randomized controlled trial. SETTING Level III mixed medical and surgical intensive care unit. PATIENTS Ninety mechanically ventilated, ...

16 Perry, R. P., ibid., 20, 216 (1960). 17 Feinendegen, L. E., V. P. Bond, and R. B. Painter, ibid., 22, 381 (1961). 18 Woods, P. S., and J. H. Taylor, Lab. Investigation, 8, 309 (1959). 19 Goldstein, L., and J. Micou, J. Biochem. Biophys. Cyt., 6, 301 (1959). 20 Pelling, G., Nature, 184, 655 (1959). 21 King, R. C., and G. J. Falk, J. Biochem. Biophys. Cyt., 8, 550 (1960). 22 Woods, P. S., Broo...