The effect of polyadenylic acid:polyuridylic acid complex (poly A:U) on immunization was investigated in mice vaccinated by killed Brucella melitensis cells suspended in incomplete adjuvant or in saline. Addition of 300 mug of poly A:U to vaccines rendered 3 x 10(8)B. melitensis cells in saline as immunogenic as 3 x 10(11) cells in oil adjuvant against a severe B. abortus challenge. In the mous...

Strains of Brucella melitensis biovars 1, 2, and 3 isolated in India, Italy, Kuwait, Saudi Arabia, the Federal Republic of Germany, and Zimbabwe were inhibited by thionin (20 micrograms/ml) but not by basic fuchsin (20 micrograms/ml) or safranin O (200 micrograms/ml). In other respects the strains were typical of their species and biovars. The existence of dye-sensitive strains of B. melitensis...

A total of 167 blood samples from aborting sheep were collected from nine different villages and the serum samples were tested for the presence of Brucella melitensis antibodies by a serum agglutination test and Chlamydophila abortus antibodies by ELISA. Seventy-one (40.11%) and nine (5.38%) serum samples were found to be positive for B. melitensis and C. abortus, respectively. These results ma...

In an enzyme-linked immunosorbent assay (ELISA), purified Brucella abortus and Escherichia coli peptidoglycan-linked lipoproteins gave a strong cross-reaction with sera from rabbits hyperimmunized with the heterologous lipoprotein. When smooth E. coli cells were used as ELISA antigens, the immunological cross-reaction was not observed unless the cells were treated to remove lipopolysaccharide a...

During the last several years, brucellosis has become an important public-health problem on a large territory part of Bosnia and Herzegovina. The disease belongs to the zoonosis group, and can be caused by several bacterium species from Brucella genus. For human and veterinarian medicine, B. abortus, B. melitensis, B. suis and B. canis from Brucella genus are important, while other brucella spe...

Hot phenol-water extraction of smooth Brucella abortus and B. melitensis cells yielded a toxic fraction which was recovered from the phenol phase (fraction 5). Chemically, fractions 5 from both Brucella species were lipid-carbohydrate-protein-2 keto-3-deoxyoctulosonic acid complexes which were stable to heat and resistant to Pronase digestion. Electron micrographs of the Brucella toxins were mo...

Brucellosis is endemic in most parts of Egypt, where it is caused mainly by Brucella melitensis biovar 3, and affects cattle and small ruminants in spite of ongoing efforts devoted to its control. Knowledge of the predominant Brucella species/strains circulating in a region is a prerequisite of a brucellosis control strategy. For this reason a study aiming at the evaluation of the phenotypic an...

results we found 28 cases with positive results for b. melitensis by multiplex pcr technique which was significantly different from of sat (p<0.05). six samples were positive for b. abortus by pcr. conclusion the results of present study showed that multiplex pcr assay is a rapid and sensitive technique for diagnosis of brucellosis compared to sat. however it is more accurate when coupled with ...

Brucellosis is a zoonotic disease of major concern in Kuwait and the Middle East. Human brucellosis can be caused by several Brucella species with varying degree of pathogenesis, and relapses are common after apparently successful therapy. The classical biochemical methods for identification of Brucella are time-consuming, cumbersome, and provide information limited to the species level only. I...

We investigated Brucella melitensis methionyl-tRNA-synthetase (BmMetRS) with molecular, structural and phenotypic methods to learn if BmMetRS is a promising target for brucellosis drug development. Recombinant BmMetRS was expressed, purified from wild type Brucella melitensis biovar Abortus 2308 strain ATCC/CRP #DD-156 and screened by a thermal melt assay against a focused library of one hundre...