Proteins that are produced for commercial purposes in Bacillus subtilis are commonly secreted via the Sec pathway. Despite its high secretion capacity, the secretion of heterologous proteins via the Sec pathway is often unsuccessful. Alternative secretion routes, like the Tat pathway, are therefore of interest. Two parallel Tat pathways with distinct specificities have previously been discovere...

The aim of this study was to isolate and express the randomly mutated α-amylase gene from B. subtilis strain 168. BS168F: 5'-gtgtcaagaatgtttgc-3' and BS168R: 3'-gttttgttaaaagatga-5' primers were used to amplify the amylase gene using the following cycle in error-prone PCR method: 94°C for 30 s, 40°C for 2 min, and 72°C for 2 min in 30 cycles that were followed with 72°C for 2 min as a post cycl...

The reversibility of thermal denaturation and catalytic efficiency of Bacillus licheniformis α-amylase were improved through site-directed mutagenesis. By using multiple sequence alignment and PoPMuSiC algorithm, Ser187 and Asn188, which located within a long loop in Domain B of Bacillus licheniformis α-amylase, were selected for mutation. In addition, Ala269, which is adjacent to Ser187 and As...

BACKGROUND Amylases are among the main enzymes used in food and other industries. They hydrolyse starch molecules into polymers composing glucose units. Amylases have potential applications in a number of industrial processes including foods and pharmaceutical industries. Alkaline α-amylase has the potential of hydrolysing starch under alkaline pH and is useful in the starch and textile industr...

Bacillus subtilis oc-amylase is inactivated by treatment with N-acetylimidazole. Full catalytic activity is restored when the acetylated protein is treated with hydroxylamine. Spectral changes associated with the loss and recovery of activity indicate that exposed residues of tyrosine react. I f the enzyme is acetylated in the presence of substrate (soluble starch) the modified protein retains ...

Bacillus amyloliquefaciens strain JJC33M is a bacterium that produces α-amylase (EC 3.2.1.1) and was isolated from sugarcane soil. Its estimated genome size is 3.96 Mb, and it harbors 4,048 coding genes (CDSs).

Endophytes are important microorganisms that enhance the plant's stability through a symbiotic relationship, without any harmful effects and symptoms in host plant. To study effect of endophytes on overall performance COH(M)8 hybrid maize seeds, present was conducted with different endophytic seed priming for 12 hrs duration Beauveria bassiana @ 5% (T2), Metarhizium anisopliae (T3) Bacillus sub...